Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration
Anthers of different species of the genera Anemone, Clematis, Papaver and Nicotiana were cultured by floating on a liquid medium which overlay an agarified charcoal medium . This technique proved to be superior to conventional methods i.e. culture on either solid or liquid media. Cold treatment of A...
Published in: | Physiologia Plantarum |
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Language: | English |
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Wiley
1982
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Online Access: | http://dx.doi.org/10.1111/j.1399-3054.1982.tb00571.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1982.tb00571.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1982.tb00571.x |
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crwiley:10.1111/j.1399-3054.1982.tb00571.x 2024-06-02T08:12:51+00:00 Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration Johansson, Lars Andersson, Barbro Eriksson, Tage 1982 http://dx.doi.org/10.1111/j.1399-3054.1982.tb00571.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1982.tb00571.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1982.tb00571.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Physiologia Plantarum volume 54, issue 1, page 24-30 ISSN 0031-9317 1399-3054 journal-article 1982 crwiley https://doi.org/10.1111/j.1399-3054.1982.tb00571.x 2024-05-03T11:37:22Z Anthers of different species of the genera Anemone, Clematis, Papaver and Nicotiana were cultured by floating on a liquid medium which overlay an agarified charcoal medium . This technique proved to be superior to conventional methods i.e. culture on either solid or liquid media. Cold treatment of Anemone anthers for 7 days after inoculation on the double layer medium gave about the same frequency of embryos per anther as corresponding cultures cold treated before inoculation. An elevation of the CO 2 concentration to 2% stimulated embryogenesis in anther cultures of Anemone canadensis, Anemone vitifolia, Papaver setigerum and Papaver radicatum . Cold treatment of cultures of Anemone canadensis inhibited embryogenesis if the ensuing culture was performed in 2% CO 2. On the other hand, cold treatment was stimulating, with an optimum of about 20 days, if the cultures were maintained in normal air. Chemical analysis of untreated anthers of Anemone canadensis showed the presence of abscisic acid (2.2 × 10 −6 g/g anthers). Cold treatment reduced the concentration of abscisic acid to 0.6 × 10 −6 g/g anthers. By use of assays with Lemna gibba as test organism, activated charcoal was shown to adsorb abscisic acid that was added to the medium. Medium treated with charcoal before inoculation of anthers of Anemone canadensis provided to inhibit embryo production. Article in Journal/Newspaper Papaver radicatum Wiley Online Library Gibba ENVELOPE(6.000,6.000,62.567,62.567) Physiologia Plantarum 54 1 24 30 |
institution |
Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Anthers of different species of the genera Anemone, Clematis, Papaver and Nicotiana were cultured by floating on a liquid medium which overlay an agarified charcoal medium . This technique proved to be superior to conventional methods i.e. culture on either solid or liquid media. Cold treatment of Anemone anthers for 7 days after inoculation on the double layer medium gave about the same frequency of embryos per anther as corresponding cultures cold treated before inoculation. An elevation of the CO 2 concentration to 2% stimulated embryogenesis in anther cultures of Anemone canadensis, Anemone vitifolia, Papaver setigerum and Papaver radicatum . Cold treatment of cultures of Anemone canadensis inhibited embryogenesis if the ensuing culture was performed in 2% CO 2. On the other hand, cold treatment was stimulating, with an optimum of about 20 days, if the cultures were maintained in normal air. Chemical analysis of untreated anthers of Anemone canadensis showed the presence of abscisic acid (2.2 × 10 −6 g/g anthers). Cold treatment reduced the concentration of abscisic acid to 0.6 × 10 −6 g/g anthers. By use of assays with Lemna gibba as test organism, activated charcoal was shown to adsorb abscisic acid that was added to the medium. Medium treated with charcoal before inoculation of anthers of Anemone canadensis provided to inhibit embryo production. |
format |
Article in Journal/Newspaper |
author |
Johansson, Lars Andersson, Barbro Eriksson, Tage |
spellingShingle |
Johansson, Lars Andersson, Barbro Eriksson, Tage Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
author_facet |
Johansson, Lars Andersson, Barbro Eriksson, Tage |
author_sort |
Johansson, Lars |
title |
Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
title_short |
Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
title_full |
Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
title_fullStr |
Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
title_full_unstemmed |
Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration |
title_sort |
improvement of anther culture technique: activated charcoal bound in agar medium in combination with liquid medium and elevated co 2 concentration |
publisher |
Wiley |
publishDate |
1982 |
url |
http://dx.doi.org/10.1111/j.1399-3054.1982.tb00571.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1982.tb00571.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1982.tb00571.x |
long_lat |
ENVELOPE(6.000,6.000,62.567,62.567) |
geographic |
Gibba |
geographic_facet |
Gibba |
genre |
Papaver radicatum |
genre_facet |
Papaver radicatum |
op_source |
Physiologia Plantarum volume 54, issue 1, page 24-30 ISSN 0031-9317 1399-3054 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1399-3054.1982.tb00571.x |
container_title |
Physiologia Plantarum |
container_volume |
54 |
container_issue |
1 |
container_start_page |
24 |
op_container_end_page |
30 |
_version_ |
1800759420542517248 |