Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library
Distinct enhancement of antibody‐specific clones was apparent during the screening against random peptide libraries with antigen‐specific polyclonal antibodies. Several sequence motifs obtained from these screenings were homologous with the primary sequence of myoglobin. Two of these motifs have bee...
Published in: | International Journal of Peptide and Protein Research |
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Online Access: | http://dx.doi.org/10.1111/j.1399-3011.1996.tb00866.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3011.1996.tb00866.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3011.1996.tb00866.x |
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crwiley:10.1111/j.1399-3011.1996.tb00866.x 2024-06-02T08:14:53+00:00 Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library YAO, ZHI‐JIAN CHAN, MOEY‐CHU KAO, MANDY C.C. CHUNG, MAXEY C.M. 1996 http://dx.doi.org/10.1111/j.1399-3011.1996.tb00866.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3011.1996.tb00866.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3011.1996.tb00866.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor International Journal of Peptide and Protein Research volume 48, issue 5, page 477-485 ISSN 0367-8377 journal-article 1996 crwiley https://doi.org/10.1111/j.1399-3011.1996.tb00866.x 2024-05-03T11:31:09Z Distinct enhancement of antibody‐specific clones was apparent during the screening against random peptide libraries with antigen‐specific polyclonal antibodies. Several sequence motifs obtained from these screenings were homologous with the primary sequence of myoglobin. Two of these motifs have been confirmed as antigenic determinants by competitive inhibition tests using eight‐branched synthetic peptides. One of the peptides has a sequence that corresponds to amino acid residues 42‐50, KFDRFKHLK, of the myoglobin sequence. This is a new epitope of myoglobin that is reported for the first time. The epitope is located precisely in the ‘turn’ or ‘loop’ region between helices C and D of the crystal structure of myoglobin. The second antibody binding site has a sequence of DIAAKYKELGYQG, and this is located between residues 141‐153, which is the C ‐terminus of myoglobin. This epitope encompassed two linear epitopes of myoglobin, amino acid residues 145‐151 and 147‐153, that have been reported earlier based on immunochemical characterisation of cleavage fragments of the protein. These results clearly indicate that epitope mapping using polyclonal antibodies against random peptide libraries can identify new epitopes precisely, as well as confirm epitopes of myoglobin obtained earlier using established methodologies. © Munksgaard 1996. Article in Journal/Newspaper Sperm whale Wiley Online Library International Journal of Peptide and Protein Research 48 5 477 485 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Distinct enhancement of antibody‐specific clones was apparent during the screening against random peptide libraries with antigen‐specific polyclonal antibodies. Several sequence motifs obtained from these screenings were homologous with the primary sequence of myoglobin. Two of these motifs have been confirmed as antigenic determinants by competitive inhibition tests using eight‐branched synthetic peptides. One of the peptides has a sequence that corresponds to amino acid residues 42‐50, KFDRFKHLK, of the myoglobin sequence. This is a new epitope of myoglobin that is reported for the first time. The epitope is located precisely in the ‘turn’ or ‘loop’ region between helices C and D of the crystal structure of myoglobin. The second antibody binding site has a sequence of DIAAKYKELGYQG, and this is located between residues 141‐153, which is the C ‐terminus of myoglobin. This epitope encompassed two linear epitopes of myoglobin, amino acid residues 145‐151 and 147‐153, that have been reported earlier based on immunochemical characterisation of cleavage fragments of the protein. These results clearly indicate that epitope mapping using polyclonal antibodies against random peptide libraries can identify new epitopes precisely, as well as confirm epitopes of myoglobin obtained earlier using established methodologies. © Munksgaard 1996. |
format |
Article in Journal/Newspaper |
author |
YAO, ZHI‐JIAN CHAN, MOEY‐CHU KAO, MANDY C.C. CHUNG, MAXEY C.M. |
spellingShingle |
YAO, ZHI‐JIAN CHAN, MOEY‐CHU KAO, MANDY C.C. CHUNG, MAXEY C.M. Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
author_facet |
YAO, ZHI‐JIAN CHAN, MOEY‐CHU KAO, MANDY C.C. CHUNG, MAXEY C.M. |
author_sort |
YAO, ZHI‐JIAN |
title |
Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
title_short |
Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
title_full |
Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
title_fullStr |
Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
title_full_unstemmed |
Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
title_sort |
linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1111/j.1399-3011.1996.tb00866.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3011.1996.tb00866.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3011.1996.tb00866.x |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
International Journal of Peptide and Protein Research volume 48, issue 5, page 477-485 ISSN 0367-8377 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1399-3011.1996.tb00866.x |
container_title |
International Journal of Peptide and Protein Research |
container_volume |
48 |
container_issue |
5 |
container_start_page |
477 |
op_container_end_page |
485 |
_version_ |
1800738893490814976 |