Xbal polymorphism of the apolipoprotein B gene influences plasma lipid response to diet intervention

• Published in: Clinical Genetics
• Main Authors: Tikkanen, M. J., Xu, C‐F., Hämäläinen, T., Talmud, P., Sarna, S., Huttunen, J. K., Pietinen, P., Humphries, S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1990
• Subjects: karelians
• Online Access: http://dx.doi.org/10.1111/j.1399-0004.1990.tb03514.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-0004.1990.tb03514.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-0004.1990.tb03514.x

Fresh blood samples were collected from 103 North Karelians who had in 1981–84 participated in dietary intervention studies and analysis of the Xbal restriction fragment length polymorphism (RFLP) of apolipoprotein B (apoB) was carried out. Reanalysis of the original plasma lipid and apolipoprotein data indicated that while baseline concentrations did not differ significantly between genotypes, the response to a low‐fat, low‐cholesterol diet was influenced by apoB Xbal genotype: reductions in total and low density lipoprotein (LDL) cholesterol, apoB and high density lipoprotein (HDL) cholesterol were greater in subjects homo‐ or heterozygous for the presence of the Xbal cutting site (X1X2 or X2X2 genotype, designated X2+) as compared to those lacking the cutting site (X1X1 genotype, designated X2—). The corresponding average reductions induced by dietary intervention in X2+ and X2— subjects were: for total cholesterol 1.30 and 0.99 mmol/1 (p=0.036), for LDL cholesterol 1.04 and 0.78 mmol/1 (p=0.049), for apoB 18.3 and 8.1 mg/100 ml (p=0.012) and for HDL cholesterol 0.26 and 0.17 mmol/1 (p = 0.008).