Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach
IgE‐antibody reactivity to boiled Atlantic shrimp ( Pandalus borealis ) and German cockroach ( Blattella germanica ) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP‐FEIA System, Pharmacia, Sweden). IgE serum leve...
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crwiley:10.1111/j.1398-9995.1995.tb02499.x 2024-06-02T08:12:51+00:00 Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach Crespo, J. F. Pascual, C. Helm, R. Sanchez‐Pastor, S. Ojeda, I. Romualdo, L. Martin‐Esteban, M. Ojeda, J. A. 1995 http://dx.doi.org/10.1111/j.1398-9995.1995.tb02499.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1398-9995.1995.tb02499.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1398-9995.1995.tb02499.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Allergy volume 50, issue 11, page 918-924 ISSN 0105-4538 1398-9995 journal-article 1995 crwiley https://doi.org/10.1111/j.1398-9995.1995.tb02499.x 2024-05-03T10:51:20Z IgE‐antibody reactivity to boiled Atlantic shrimp ( Pandalus borealis ) and German cockroach ( Blattella germanica ) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP‐FEIA System, Pharmacia, Sweden). IgE serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. A positive anticockroach IgE was very rare in the absence of detectable antishrimp IgE (five of 89 sera). Linear regression analysis on antishrimp and anti‐German cockroach IgE levels‐log plot revealed correlation coefficient (r) of 0.73. Inhibition experiments showed that boiled Atlantic shrimp extract inhibited CAP with German cockroach, and vice versa. Immunoblotting showed the strongest IgE binding for both allergenic extracts between 30 and 43 kDa. By blot inhibition, the binding capacity of German cockroach was totally‐abolished by Atlantic shrimp extract, while German cockroach extract only partially IgE binding to Atlantic shrimp. Cross‐reactivity exists between shrimp, an important food allergen, and German cockroach, which has an increasing role in allergic asthma. It could be important to determine the clinical significance of cross‐allergy to both allergens, in which exposures occur in different ways. Article in Journal/Newspaper Pandalus borealis Wiley Online Library Allergy 50 11 918 924 |
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Wiley Online Library |
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English |
description |
IgE‐antibody reactivity to boiled Atlantic shrimp ( Pandalus borealis ) and German cockroach ( Blattella germanica ) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP‐FEIA System, Pharmacia, Sweden). IgE serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. A positive anticockroach IgE was very rare in the absence of detectable antishrimp IgE (five of 89 sera). Linear regression analysis on antishrimp and anti‐German cockroach IgE levels‐log plot revealed correlation coefficient (r) of 0.73. Inhibition experiments showed that boiled Atlantic shrimp extract inhibited CAP with German cockroach, and vice versa. Immunoblotting showed the strongest IgE binding for both allergenic extracts between 30 and 43 kDa. By blot inhibition, the binding capacity of German cockroach was totally‐abolished by Atlantic shrimp extract, while German cockroach extract only partially IgE binding to Atlantic shrimp. Cross‐reactivity exists between shrimp, an important food allergen, and German cockroach, which has an increasing role in allergic asthma. It could be important to determine the clinical significance of cross‐allergy to both allergens, in which exposures occur in different ways. |
format |
Article in Journal/Newspaper |
author |
Crespo, J. F. Pascual, C. Helm, R. Sanchez‐Pastor, S. Ojeda, I. Romualdo, L. Martin‐Esteban, M. Ojeda, J. A. |
spellingShingle |
Crespo, J. F. Pascual, C. Helm, R. Sanchez‐Pastor, S. Ojeda, I. Romualdo, L. Martin‐Esteban, M. Ojeda, J. A. Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
author_facet |
Crespo, J. F. Pascual, C. Helm, R. Sanchez‐Pastor, S. Ojeda, I. Romualdo, L. Martin‐Esteban, M. Ojeda, J. A. |
author_sort |
Crespo, J. F. |
title |
Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
title_short |
Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
title_full |
Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
title_fullStr |
Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
title_full_unstemmed |
Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach |
title_sort |
cross‐reactivity of ige‐binding components between boiled atlantic shrimp and german cockroach |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1111/j.1398-9995.1995.tb02499.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1398-9995.1995.tb02499.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1398-9995.1995.tb02499.x |
genre |
Pandalus borealis |
genre_facet |
Pandalus borealis |
op_source |
Allergy volume 50, issue 11, page 918-924 ISSN 0105-4538 1398-9995 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1398-9995.1995.tb02499.x |
container_title |
Allergy |
container_volume |
50 |
container_issue |
11 |
container_start_page |
918 |
op_container_end_page |
924 |
_version_ |
1800759412784103424 |