The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma
Abstract Background In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5‐2), against murine UV‐induced 2237 fibrosarcoma and B16F10 melanoma cells. Methods The 2237,...
Published in: | International Journal of Dermatology |
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Online Access: | http://dx.doi.org/10.1111/j.1365-4632.2010.04825.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-4632.2010.04825.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-4632.2010.04825.x |
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crwiley:10.1111/j.1365-4632.2010.04825.x 2024-06-02T07:58:29+00:00 The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma Mojib, Nazia Nasti, Tahseen H. Andersen, Dale T. Attigada, Venkatram R. Hoover, Richard B. Yusuf, Nabiha Bej, Asim K. 2011 http://dx.doi.org/10.1111/j.1365-4632.2010.04825.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-4632.2010.04825.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-4632.2010.04825.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor International Journal of Dermatology volume 50, issue 10, page 1223-1233 ISSN 0011-9059 1365-4632 journal-article 2011 crwiley https://doi.org/10.1111/j.1365-4632.2010.04825.x 2024-05-03T10:43:07Z Abstract Background In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5‐2), against murine UV‐induced 2237 fibrosarcoma and B16F10 melanoma cells. Methods The 2237, B16F10, C50, and NIH3T3 cells were treated with PVP at different doses and for different times, and their proliferation and viability were detected by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced by PVP in 2237 fibrosarcoma cells was assessed by flow cytometry and expression analysis of cell cycle regulatory proteins were done by Western blot. Apoptosis induced by PVP in 2237 cells was observed by annexin‐V/propidium iodide double staining flow cytometry assay and fluorescence microscopy. To further determine the molecular mechanism of apoptosis induced by PVP, the changes in expression of Bcl‐2, Bax and cytochrome c were detected by Western blot. The loss of mitochondrial membrane potential in PVP treated 2237 cells was assessed by staining with JC‐1 dye following flow cytometry. Caspase‐3, Caspase‐9 and PARP cleavage were analyzed by Western blot and Caspase‐3 and ‐9 activities were measured by colorimetric assays. Results In vitro treatment of murine 2237 cells with the PVP resulted in decreased cell viability (13–79%) in a time (24–72 h) and dose (0.1–1 μ m )‐dependent manner. The PVP‐induced growth inhibition in 2237 cells was associated with both G0/G1 and G2/M phase arrest accompanied with decrease in the expression of cyclin dependent kinases (Cdks) and simultaneous increase in the expression of cyclin dependent kinase inhibitors (Cdki) – Cip1/p21 and Kip1/p27. Further, we observed a significant increase in the apoptosis of the 2237 fibrosarcoma cells which was associated with an increased expression of pro‐apoptotic protein Bax, decreased expression of anti‐apoptotic proteins Bcl‐2, disruption of mitochondrial membrane potential, cytochrome c release, ... Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic International Journal of Dermatology 50 10 1223 1233 |
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Wiley Online Library |
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English |
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Abstract Background In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5‐2), against murine UV‐induced 2237 fibrosarcoma and B16F10 melanoma cells. Methods The 2237, B16F10, C50, and NIH3T3 cells were treated with PVP at different doses and for different times, and their proliferation and viability were detected by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced by PVP in 2237 fibrosarcoma cells was assessed by flow cytometry and expression analysis of cell cycle regulatory proteins were done by Western blot. Apoptosis induced by PVP in 2237 cells was observed by annexin‐V/propidium iodide double staining flow cytometry assay and fluorescence microscopy. To further determine the molecular mechanism of apoptosis induced by PVP, the changes in expression of Bcl‐2, Bax and cytochrome c were detected by Western blot. The loss of mitochondrial membrane potential in PVP treated 2237 cells was assessed by staining with JC‐1 dye following flow cytometry. Caspase‐3, Caspase‐9 and PARP cleavage were analyzed by Western blot and Caspase‐3 and ‐9 activities were measured by colorimetric assays. Results In vitro treatment of murine 2237 cells with the PVP resulted in decreased cell viability (13–79%) in a time (24–72 h) and dose (0.1–1 μ m )‐dependent manner. The PVP‐induced growth inhibition in 2237 cells was associated with both G0/G1 and G2/M phase arrest accompanied with decrease in the expression of cyclin dependent kinases (Cdks) and simultaneous increase in the expression of cyclin dependent kinase inhibitors (Cdki) – Cip1/p21 and Kip1/p27. Further, we observed a significant increase in the apoptosis of the 2237 fibrosarcoma cells which was associated with an increased expression of pro‐apoptotic protein Bax, decreased expression of anti‐apoptotic proteins Bcl‐2, disruption of mitochondrial membrane potential, cytochrome c release, ... |
format |
Article in Journal/Newspaper |
author |
Mojib, Nazia Nasti, Tahseen H. Andersen, Dale T. Attigada, Venkatram R. Hoover, Richard B. Yusuf, Nabiha Bej, Asim K. |
spellingShingle |
Mojib, Nazia Nasti, Tahseen H. Andersen, Dale T. Attigada, Venkatram R. Hoover, Richard B. Yusuf, Nabiha Bej, Asim K. The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
author_facet |
Mojib, Nazia Nasti, Tahseen H. Andersen, Dale T. Attigada, Venkatram R. Hoover, Richard B. Yusuf, Nabiha Bej, Asim K. |
author_sort |
Mojib, Nazia |
title |
The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
title_short |
The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
title_full |
The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
title_fullStr |
The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
title_full_unstemmed |
The antiproliferative function of violacein‐like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5‐2 in UV‐induced 2237 fibrosarcoma |
title_sort |
antiproliferative function of violacein‐like purple violet pigment (pvp) from an antarctic janthinobacterium sp. ant5‐2 in uv‐induced 2237 fibrosarcoma |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1365-4632.2010.04825.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-4632.2010.04825.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-4632.2010.04825.x |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Dermatology volume 50, issue 10, page 1223-1233 ISSN 0011-9059 1365-4632 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-4632.2010.04825.x |
container_title |
International Journal of Dermatology |
container_volume |
50 |
container_issue |
10 |
container_start_page |
1223 |
op_container_end_page |
1233 |
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1800741845158854656 |