Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites
Abstract The response of the kinetic properties of NAD malate dehydrogenase (MDH) was compared for two clones of Lathy rus japonicus Willd. collected in two contrasting climatic sites in Eastern North America. MDH from the cold adapted maritime genotype (Hudson Bay, Québec) had lower thermostability...
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Online Access: | http://dx.doi.org/10.1111/j.1365-3040.1979.tb00770.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3040.1979.tb00770.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3040.1979.tb00770.x |
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crwiley:10.1111/j.1365-3040.1979.tb00770.x 2024-06-02T08:07:53+00:00 Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites SIMON, JEAN‐PIERRE 1979 http://dx.doi.org/10.1111/j.1365-3040.1979.tb00770.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3040.1979.tb00770.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3040.1979.tb00770.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Plant, Cell & Environment volume 2, issue 1, page 23-33 ISSN 0140-7791 1365-3040 journal-article 1979 crwiley https://doi.org/10.1111/j.1365-3040.1979.tb00770.x 2024-05-03T11:33:23Z Abstract The response of the kinetic properties of NAD malate dehydrogenase (MDH) was compared for two clones of Lathy rus japonicus Willd. collected in two contrasting climatic sites in Eastern North America. MDH from the cold adapted maritime genotype (Hudson Bay, Québec) had lower thermostability, reduced apparent (E a ) and free (ΔG † ) energy of activation and lower specific activity when compared to MDH of plants from the warm summer continental site (Lake Michigan). Electrophoretic analyses show little differentiation in the isozyme profiles of the two genotypes. Thermostability differences were primarily associated with the mitochondrial isozymes which, however, were not differentiated electrophoretically Substrate binding ability of MDH, as measured by apparent Km, was more sensitive to high assay temperature in the cold adapted maritime genotype. Article in Journal/Newspaper Hudson Bay Wiley Online Library Hudson Hudson Bay Rus’ ENVELOPE(155.950,155.950,54.200,54.200) Plant, Cell & Environment 2 1 23 33 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The response of the kinetic properties of NAD malate dehydrogenase (MDH) was compared for two clones of Lathy rus japonicus Willd. collected in two contrasting climatic sites in Eastern North America. MDH from the cold adapted maritime genotype (Hudson Bay, Québec) had lower thermostability, reduced apparent (E a ) and free (ΔG † ) energy of activation and lower specific activity when compared to MDH of plants from the warm summer continental site (Lake Michigan). Electrophoretic analyses show little differentiation in the isozyme profiles of the two genotypes. Thermostability differences were primarily associated with the mitochondrial isozymes which, however, were not differentiated electrophoretically Substrate binding ability of MDH, as measured by apparent Km, was more sensitive to high assay temperature in the cold adapted maritime genotype. |
format |
Article in Journal/Newspaper |
author |
SIMON, JEAN‐PIERRE |
spellingShingle |
SIMON, JEAN‐PIERRE Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
author_facet |
SIMON, JEAN‐PIERRE |
author_sort |
SIMON, JEAN‐PIERRE |
title |
Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
title_short |
Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
title_full |
Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
title_fullStr |
Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
title_full_unstemmed |
Differences in thermal properties of NAD malate dehydrogenase in genotypes of Lathyrus japonicus Willd (Leguminosae) from maritime and continental sites |
title_sort |
differences in thermal properties of nad malate dehydrogenase in genotypes of lathyrus japonicus willd (leguminosae) from maritime and continental sites |
publisher |
Wiley |
publishDate |
1979 |
url |
http://dx.doi.org/10.1111/j.1365-3040.1979.tb00770.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3040.1979.tb00770.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3040.1979.tb00770.x |
long_lat |
ENVELOPE(155.950,155.950,54.200,54.200) |
geographic |
Hudson Hudson Bay Rus’ |
geographic_facet |
Hudson Hudson Bay Rus’ |
genre |
Hudson Bay |
genre_facet |
Hudson Bay |
op_source |
Plant, Cell & Environment volume 2, issue 1, page 23-33 ISSN 0140-7791 1365-3040 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-3040.1979.tb00770.x |
container_title |
Plant, Cell & Environment |
container_volume |
2 |
container_issue |
1 |
container_start_page |
23 |
op_container_end_page |
33 |
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1800753022574264320 |