Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation
SUMMARY We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E 2 (PGE 2 ) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE 2 , the immunomodulatory activity of unfractiona...
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crwiley:10.1111/j.1365-3024.2007.00932.x 2024-09-15T17:55:39+00:00 Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation FAST, M. D. JOHNSON, S. C. EDDY, T. D. PINTO, D. ROSS, N. W. 2007 http://dx.doi.org/10.1111/j.1365-3024.2007.00932.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3024.2007.00932.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3024.2007.00932.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Parasite Immunology volume 29, issue 4, page 179-189 ISSN 0141-9838 1365-3024 journal-article 2007 crwiley https://doi.org/10.1111/j.1365-3024.2007.00932.x 2024-08-01T04:19:43Z SUMMARY We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E 2 (PGE 2 ) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE 2 , the immunomodulatory activity of unfractionated and pools of the fractionated secretions was determined by examining the effects of the secretions on Atlantic salmon immune gene expression. Incubation of macrophage‐enriched isolates of Atlantic salmon head kidney cells with the unfractionated secretion + PGE 2 revealed a significant inhibition of interleukin‐1β (IL‐1β) and major histocompatibility class I gene expression. Inhibition of lipopolysaccharide‐induced IL‐1β expression in the Atlantic salmon head kidney cell line (SHK‐1) was observed when three pools of the secretory/excretory products were tested. Further purification of products within these pools revealed that fraction 1‐2 could account fully for the inhibition of IL‐1β expression in SHK‐1 cells observed in pooled fraction 1. This study demonstrates that there are other immunomodulatory compounds produced by L. salmonis , in addition to PGE 2 and trypsin, that can inhibit the expression of Atlantic salmon immune‐related genes in vitro . Article in Journal/Newspaper Atlantic salmon Wiley Online Library Parasite Immunology 29 4 179 189 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
SUMMARY We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E 2 (PGE 2 ) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE 2 , the immunomodulatory activity of unfractionated and pools of the fractionated secretions was determined by examining the effects of the secretions on Atlantic salmon immune gene expression. Incubation of macrophage‐enriched isolates of Atlantic salmon head kidney cells with the unfractionated secretion + PGE 2 revealed a significant inhibition of interleukin‐1β (IL‐1β) and major histocompatibility class I gene expression. Inhibition of lipopolysaccharide‐induced IL‐1β expression in the Atlantic salmon head kidney cell line (SHK‐1) was observed when three pools of the secretory/excretory products were tested. Further purification of products within these pools revealed that fraction 1‐2 could account fully for the inhibition of IL‐1β expression in SHK‐1 cells observed in pooled fraction 1. This study demonstrates that there are other immunomodulatory compounds produced by L. salmonis , in addition to PGE 2 and trypsin, that can inhibit the expression of Atlantic salmon immune‐related genes in vitro . |
format |
Article in Journal/Newspaper |
author |
FAST, M. D. JOHNSON, S. C. EDDY, T. D. PINTO, D. ROSS, N. W. |
spellingShingle |
FAST, M. D. JOHNSON, S. C. EDDY, T. D. PINTO, D. ROSS, N. W. Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
author_facet |
FAST, M. D. JOHNSON, S. C. EDDY, T. D. PINTO, D. ROSS, N. W. |
author_sort |
FAST, M. D. |
title |
Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
title_short |
Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
title_full |
Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
title_fullStr |
Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
title_full_unstemmed |
Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation |
title_sort |
lepeophtheirus salmonis secretory/excretory products and their effects on atlantic salmon immune gene regulation |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1365-3024.2007.00932.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3024.2007.00932.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3024.2007.00932.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Parasite Immunology volume 29, issue 4, page 179-189 ISSN 0141-9838 1365-3024 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-3024.2007.00932.x |
container_title |
Parasite Immunology |
container_volume |
29 |
container_issue |
4 |
container_start_page |
179 |
op_container_end_page |
189 |
_version_ |
1810431906661007360 |