A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii
Summary A global view of the biology of the cold‐adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4°C versus T opt (23°C) were identified by mass spectrometry using the draft genome sequence of M. burtonii . mRNA levels were determined for all...
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crwiley:10.1111/j.1365-2958.2004.04130.x 2024-09-09T19:08:11+00:00 A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii Goodchild, Amber Saunders, Neil F. W. Ertan, Haluk Raftery, Mark Guilhaus, Michael Curmi, Paul M. G. Cavicchioli, Ricardo 2004 http://dx.doi.org/10.1111/j.1365-2958.2004.04130.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.2004.04130.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2004.04130.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Microbiology volume 53, issue 1, page 309-321 ISSN 0950-382X 1365-2958 journal-article 2004 crwiley https://doi.org/10.1111/j.1365-2958.2004.04130.x 2024-08-13T04:18:34Z Summary A global view of the biology of the cold‐adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4°C versus T opt (23°C) were identified by mass spectrometry using the draft genome sequence of M. burtonii . mRNA levels were determined for all genes identified by proteomics, and specific enzyme assays confirmed the protein expression results. Key aspects of cold adaptation related to transcription, protein folding and metabolism, including specific roles for RNA polymerase subunit E, a response regulator and peptidyl prolyl cis/trans isomerase. Heat shock protein DnaK was expressed during growth at T opt , indicating that growth at ‘optimal’ temperatures was stressful for this cold‐adapted organism. Expression of trimethylamine methyltransferase involves contiguous translation of two open reading frames, which is likely to result from incorporation of pyrrolysine at an amber stop codon. Thermal regulation in M. burtonii is achieved through complex gene expression events involving gene clusters and operons, through to protein modifications. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Molecular Microbiology 53 1 309 321 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary A global view of the biology of the cold‐adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4°C versus T opt (23°C) were identified by mass spectrometry using the draft genome sequence of M. burtonii . mRNA levels were determined for all genes identified by proteomics, and specific enzyme assays confirmed the protein expression results. Key aspects of cold adaptation related to transcription, protein folding and metabolism, including specific roles for RNA polymerase subunit E, a response regulator and peptidyl prolyl cis/trans isomerase. Heat shock protein DnaK was expressed during growth at T opt , indicating that growth at ‘optimal’ temperatures was stressful for this cold‐adapted organism. Expression of trimethylamine methyltransferase involves contiguous translation of two open reading frames, which is likely to result from incorporation of pyrrolysine at an amber stop codon. Thermal regulation in M. burtonii is achieved through complex gene expression events involving gene clusters and operons, through to protein modifications. |
format |
Article in Journal/Newspaper |
author |
Goodchild, Amber Saunders, Neil F. W. Ertan, Haluk Raftery, Mark Guilhaus, Michael Curmi, Paul M. G. Cavicchioli, Ricardo |
spellingShingle |
Goodchild, Amber Saunders, Neil F. W. Ertan, Haluk Raftery, Mark Guilhaus, Michael Curmi, Paul M. G. Cavicchioli, Ricardo A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
author_facet |
Goodchild, Amber Saunders, Neil F. W. Ertan, Haluk Raftery, Mark Guilhaus, Michael Curmi, Paul M. G. Cavicchioli, Ricardo |
author_sort |
Goodchild, Amber |
title |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_short |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_full |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_fullStr |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_full_unstemmed |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_sort |
proteomic determination of cold adaptation in the antarctic archaeon, methanococcoides burtonii |
publisher |
Wiley |
publishDate |
2004 |
url |
http://dx.doi.org/10.1111/j.1365-2958.2004.04130.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.2004.04130.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2004.04130.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Molecular Microbiology volume 53, issue 1, page 309-321 ISSN 0950-382X 1365-2958 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2958.2004.04130.x |
container_title |
Molecular Microbiology |
container_volume |
53 |
container_issue |
1 |
container_start_page |
309 |
op_container_end_page |
321 |
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1809822390818963456 |