Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing
Abstract We investigated changes in the root‐associated fungal communities associated with the ectomycorrhizal herb Bistorta vivipara along a primary succession gradient using 454 amplicon sequencing. Our main objective was to assess the degree of variation in fungal richness and community compositi...
Published in: | Molecular Ecology |
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crwiley:10.1111/j.1365-294x.2011.05214.x 2024-06-23T07:53:04+00:00 Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing BLAALID, RAKEL CARLSEN, TOR KUMAR, SURENDRA HALVORSEN, RUNE UGLAND, KARL INNE FONTANA, GIOVANNI KAUSERUD, HÅVARD 2011 http://dx.doi.org/10.1111/j.1365-294x.2011.05214.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.2011.05214.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.2011.05214.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 21, issue 8, page 1897-1908 ISSN 0962-1083 1365-294X journal-article 2011 crwiley https://doi.org/10.1111/j.1365-294x.2011.05214.x 2024-06-06T04:22:28Z Abstract We investigated changes in the root‐associated fungal communities associated with the ectomycorrhizal herb Bistorta vivipara along a primary succession gradient using 454 amplicon sequencing. Our main objective was to assess the degree of variation in fungal richness and community composition as vegetation cover increases along the chronosequence. Sixty root systems of B. vivipara were sampled in vegetation zones delimited by dated moraines in front of a retreating glacier in Norway. We extracted DNA from rinsed root systems, amplified the ITS1 region using fungal‐specific primers and analysed the amplicons using 454 sequencing. Between 437 and 5063 sequences were obtained from each root system. Clustering analyses using a 98.5% sequence similarity cut‐off yielded a total of 470 operational taxonomic units (OTUs), excluding singletons. Between eight and 41 fungal OTUs were detected within each root system. Already in the first stage of succession, a high fungal diversity was present in the B. vivipara root systems. Total number of OTUs increased significantly along the gradient towards climax vegetation, but the average number of OTUs per root system stayed unchanged. There was a high patchiness in distribution of fungal OTUs across root systems, indicating that stochastic processes to a large extent structure the fungal communities. However, time since deglaciation had impact on the fungal community structure, as a systematic shift in the community composition was observed along the chronosequence. Ectomycorrhizal basidiomycetes were the dominant fungi in the roots of B. vivipara, when it comes to both number of OTUs and number of sequences. Article in Journal/Newspaper glacier Wiley Online Library Norway Molecular Ecology 21 8 1897 1908 |
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English |
description |
Abstract We investigated changes in the root‐associated fungal communities associated with the ectomycorrhizal herb Bistorta vivipara along a primary succession gradient using 454 amplicon sequencing. Our main objective was to assess the degree of variation in fungal richness and community composition as vegetation cover increases along the chronosequence. Sixty root systems of B. vivipara were sampled in vegetation zones delimited by dated moraines in front of a retreating glacier in Norway. We extracted DNA from rinsed root systems, amplified the ITS1 region using fungal‐specific primers and analysed the amplicons using 454 sequencing. Between 437 and 5063 sequences were obtained from each root system. Clustering analyses using a 98.5% sequence similarity cut‐off yielded a total of 470 operational taxonomic units (OTUs), excluding singletons. Between eight and 41 fungal OTUs were detected within each root system. Already in the first stage of succession, a high fungal diversity was present in the B. vivipara root systems. Total number of OTUs increased significantly along the gradient towards climax vegetation, but the average number of OTUs per root system stayed unchanged. There was a high patchiness in distribution of fungal OTUs across root systems, indicating that stochastic processes to a large extent structure the fungal communities. However, time since deglaciation had impact on the fungal community structure, as a systematic shift in the community composition was observed along the chronosequence. Ectomycorrhizal basidiomycetes were the dominant fungi in the roots of B. vivipara, when it comes to both number of OTUs and number of sequences. |
format |
Article in Journal/Newspaper |
author |
BLAALID, RAKEL CARLSEN, TOR KUMAR, SURENDRA HALVORSEN, RUNE UGLAND, KARL INNE FONTANA, GIOVANNI KAUSERUD, HÅVARD |
spellingShingle |
BLAALID, RAKEL CARLSEN, TOR KUMAR, SURENDRA HALVORSEN, RUNE UGLAND, KARL INNE FONTANA, GIOVANNI KAUSERUD, HÅVARD Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
author_facet |
BLAALID, RAKEL CARLSEN, TOR KUMAR, SURENDRA HALVORSEN, RUNE UGLAND, KARL INNE FONTANA, GIOVANNI KAUSERUD, HÅVARD |
author_sort |
BLAALID, RAKEL |
title |
Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
title_short |
Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
title_full |
Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
title_fullStr |
Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
title_full_unstemmed |
Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
title_sort |
changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1365-294x.2011.05214.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.2011.05214.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.2011.05214.x |
geographic |
Norway |
geographic_facet |
Norway |
genre |
glacier |
genre_facet |
glacier |
op_source |
Molecular Ecology volume 21, issue 8, page 1897-1908 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/j.1365-294x.2011.05214.x |
container_title |
Molecular Ecology |
container_volume |
21 |
container_issue |
8 |
container_start_page |
1897 |
op_container_end_page |
1908 |
_version_ |
1802644546135261184 |