An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species
Abstract The dating of recent events in the history of organisms needs divergence rates based on molecular fingerprint markers. Here, we used amplified fragment length polymorphisms (AFLPs) of three distantly related alpine plant species co‐occurring in the Spanish Sierra Nevada, the Pyrenees and th...
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Online Access: | http://dx.doi.org/10.1111/j.1365-294x.2008.04053.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.2008.04053.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.2008.04053.x |
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crwiley:10.1111/j.1365-294x.2008.04053.x 2024-06-02T08:15:06+00:00 An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species KROPF, MATTHIAS COMES, HANS PETER KADEREIT, JOACHIM W. 2009 http://dx.doi.org/10.1111/j.1365-294x.2008.04053.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.2008.04053.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.2008.04053.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 18, issue 4, page 697-708 ISSN 0962-1083 1365-294X journal-article 2009 crwiley https://doi.org/10.1111/j.1365-294x.2008.04053.x 2024-05-03T11:24:01Z Abstract The dating of recent events in the history of organisms needs divergence rates based on molecular fingerprint markers. Here, we used amplified fragment length polymorphisms (AFLPs) of three distantly related alpine plant species co‐occurring in the Spanish Sierra Nevada, the Pyrenees and the southwestern Alps/Massif Central to establish divergence rates. Within each of these species ( Gentiana alpina , Kernera saxatilis and Silene rupestris ), we found that the degree of AFLP divergence ( D N72 ) between mountain phylogroups was significantly correlated with their time of divergence (as inferred from palaeoclimatic/palynological data), indicating constant AFLP divergence rates. As these rates did not differ significantly among species, a regression analysis based on the pooled data was utilized to generate a general AFLP rate. The application of this latter rate to AFLP data from other herbaceous plant species ( Minuartia biflora : Schönswetter et al . 2006 Nigella degenii : Comes et al . 2008 ) resulted in a plausible timing of the recolonization of the Svalbard Islands and the separation of populations from the Alps and Scandinavia ( Minuartia ), and of island population separation in the Aegean Archipelago ( Nigella ). Furthermore, the AFLP mutation rate obtained in our study is of the same magnitude as AFLP mutation rates published previously. The temporal limits of our AFLP rate, which is based on intraspecific vicariance events at shallow (i.e. late glacial/Early Holocene) time scales, remains to be tested. Article in Journal/Newspaper Svalbard Wiley Online Library Svalbard Molecular Ecology 18 4 697 708 |
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English |
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Abstract The dating of recent events in the history of organisms needs divergence rates based on molecular fingerprint markers. Here, we used amplified fragment length polymorphisms (AFLPs) of three distantly related alpine plant species co‐occurring in the Spanish Sierra Nevada, the Pyrenees and the southwestern Alps/Massif Central to establish divergence rates. Within each of these species ( Gentiana alpina , Kernera saxatilis and Silene rupestris ), we found that the degree of AFLP divergence ( D N72 ) between mountain phylogroups was significantly correlated with their time of divergence (as inferred from palaeoclimatic/palynological data), indicating constant AFLP divergence rates. As these rates did not differ significantly among species, a regression analysis based on the pooled data was utilized to generate a general AFLP rate. The application of this latter rate to AFLP data from other herbaceous plant species ( Minuartia biflora : Schönswetter et al . 2006 Nigella degenii : Comes et al . 2008 ) resulted in a plausible timing of the recolonization of the Svalbard Islands and the separation of populations from the Alps and Scandinavia ( Minuartia ), and of island population separation in the Aegean Archipelago ( Nigella ). Furthermore, the AFLP mutation rate obtained in our study is of the same magnitude as AFLP mutation rates published previously. The temporal limits of our AFLP rate, which is based on intraspecific vicariance events at shallow (i.e. late glacial/Early Holocene) time scales, remains to be tested. |
format |
Article in Journal/Newspaper |
author |
KROPF, MATTHIAS COMES, HANS PETER KADEREIT, JOACHIM W. |
spellingShingle |
KROPF, MATTHIAS COMES, HANS PETER KADEREIT, JOACHIM W. An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
author_facet |
KROPF, MATTHIAS COMES, HANS PETER KADEREIT, JOACHIM W. |
author_sort |
KROPF, MATTHIAS |
title |
An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
title_short |
An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
title_full |
An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
title_fullStr |
An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
title_full_unstemmed |
An AFLP clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern European alpine plant species |
title_sort |
aflp clock for the absolute dating of shallow‐time evolutionary history based on the intraspecific divergence of southwestern european alpine plant species |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1365-294x.2008.04053.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.2008.04053.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.2008.04053.x |
geographic |
Svalbard |
geographic_facet |
Svalbard |
genre |
Svalbard |
genre_facet |
Svalbard |
op_source |
Molecular Ecology volume 18, issue 4, page 697-708 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-294x.2008.04053.x |
container_title |
Molecular Ecology |
container_volume |
18 |
container_issue |
4 |
container_start_page |
697 |
op_container_end_page |
708 |
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1800739188432175104 |