Sexing free‐ranging brown bears Ursus arctos using hairs found in the field
Abstract As an aid to the management of the Pyrenean population of the brown bear Ursus arctos , a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two‐step polymerase...
Published in: | Molecular Ecology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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1993
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Online Access: | http://dx.doi.org/10.1111/j.1365-294x.1993.tb00033.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.1993.tb00033.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.1993.tb00033.x |
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crwiley:10.1111/j.1365-294x.1993.tb00033.x 2024-03-31T07:55:40+00:00 Sexing free‐ranging brown bears Ursus arctos using hairs found in the field TABERLET, P. MATTOCK, H. DUBOIS‐PAGANON, C. BOUVET, J. 1993 http://dx.doi.org/10.1111/j.1365-294x.1993.tb00033.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.1993.tb00033.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.1993.tb00033.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 2, issue 6, page 399-403 ISSN 0962-1083 1365-294X Genetics Ecology, Evolution, Behavior and Systematics journal-article 1993 crwiley https://doi.org/10.1111/j.1365-294x.1993.tb00033.x 2024-03-04T13:01:59Z Abstract As an aid to the management of the Pyrenean population of the brown bear Ursus arctos , a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two‐step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals. Article in Journal/Newspaper Ursus arctos Wiley Online Library Molecular Ecology 2 6 399 403 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics TABERLET, P. MATTOCK, H. DUBOIS‐PAGANON, C. BOUVET, J. Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics |
description |
Abstract As an aid to the management of the Pyrenean population of the brown bear Ursus arctos , a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two‐step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals. |
format |
Article in Journal/Newspaper |
author |
TABERLET, P. MATTOCK, H. DUBOIS‐PAGANON, C. BOUVET, J. |
author_facet |
TABERLET, P. MATTOCK, H. DUBOIS‐PAGANON, C. BOUVET, J. |
author_sort |
TABERLET, P. |
title |
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
title_short |
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
title_full |
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
title_fullStr |
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
title_full_unstemmed |
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field |
title_sort |
sexing free‐ranging brown bears ursus arctos using hairs found in the field |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1111/j.1365-294x.1993.tb00033.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-294X.1993.tb00033.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-294X.1993.tb00033.x |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Molecular Ecology volume 2, issue 6, page 399-403 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-294x.1993.tb00033.x |
container_title |
Molecular Ecology |
container_volume |
2 |
container_issue |
6 |
container_start_page |
399 |
op_container_end_page |
403 |
_version_ |
1795037857030602752 |