Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples
Summary A new light microscope–temperature‐controlled chamber (LM–TCC) has been constructed. The special feature of the light microscope–temperature‐controlled chamber is the Peltier‐element temperature control of a specimen holder for biological samples, with a volume capacity of 1 mL. This system...
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crwiley:10.1111/j.1365-2818.2007.01730.x 2024-09-15T18:31:50+00:00 Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples BUCHNER, O. LÜTZ, C. HOLZINGER, A. 2007 http://dx.doi.org/10.1111/j.1365-2818.2007.01730.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.2007.01730.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.2007.01730.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Microscopy volume 225, issue 2, page 183-191 ISSN 0022-2720 1365-2818 journal-article 2007 crwiley https://doi.org/10.1111/j.1365-2818.2007.01730.x 2024-08-01T04:22:35Z Summary A new light microscope–temperature‐controlled chamber (LM–TCC) has been constructed. The special feature of the light microscope–temperature‐controlled chamber is the Peltier‐element temperature control of a specimen holder for biological samples, with a volume capacity of 1 mL. This system has marked advantages when compared to other approaches for temperature‐controlled microscopy. It works in a temperature range of −10°C to +95°C with an accuracy of ±0.1°C in the stationary phase. The light microscope–temperature‐controlled chamber allows rapid temperature shift rates. A maximum heating rate of 12.9°C min −1 and a maximum cooling rate of 6.0°C min −1 are achieved with minimized overshoots (≤1.9°C). This machinery operates at low cost and external coolants are not required. Especially with samples absorbing irradiation strongly, temperature control during microscopy is necessary to avoid overheating of samples. For example, leaf segments of Ficaria verna exposed to 4500 μmol photons m −2 s −1 in a standard microscopic preparation show a temperature increase (δT) of 18.0°C, whereas in the light microscope–temperature‐controlled chamber this is reduced to 4°C. The kinetics of microscope‐light induced δT are described and infrared thermography demonstrates the dissipation of the temperature. Chloroplasts of the cold adapted plant Ranunculus glacialis show the tendency to form stroma‐filled protrusions in relation to the exposure temperature. The relative number of chloroplasts with protrusions is reduced at 5°C when compared to 25°C. This effect is reversible. The new light microscope–temperature‐controlled chamber will be useful in a wide range of biological applications where a rapid change of temperature during microscopic observations is necessary or has to be avoided allowing a simulation of ecologically relevant temperature scenarios. Article in Journal/Newspaper Ranunculus glacialis Wiley Online Library Journal of Microscopy 225 2 183 191 |
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Wiley Online Library |
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English |
description |
Summary A new light microscope–temperature‐controlled chamber (LM–TCC) has been constructed. The special feature of the light microscope–temperature‐controlled chamber is the Peltier‐element temperature control of a specimen holder for biological samples, with a volume capacity of 1 mL. This system has marked advantages when compared to other approaches for temperature‐controlled microscopy. It works in a temperature range of −10°C to +95°C with an accuracy of ±0.1°C in the stationary phase. The light microscope–temperature‐controlled chamber allows rapid temperature shift rates. A maximum heating rate of 12.9°C min −1 and a maximum cooling rate of 6.0°C min −1 are achieved with minimized overshoots (≤1.9°C). This machinery operates at low cost and external coolants are not required. Especially with samples absorbing irradiation strongly, temperature control during microscopy is necessary to avoid overheating of samples. For example, leaf segments of Ficaria verna exposed to 4500 μmol photons m −2 s −1 in a standard microscopic preparation show a temperature increase (δT) of 18.0°C, whereas in the light microscope–temperature‐controlled chamber this is reduced to 4°C. The kinetics of microscope‐light induced δT are described and infrared thermography demonstrates the dissipation of the temperature. Chloroplasts of the cold adapted plant Ranunculus glacialis show the tendency to form stroma‐filled protrusions in relation to the exposure temperature. The relative number of chloroplasts with protrusions is reduced at 5°C when compared to 25°C. This effect is reversible. The new light microscope–temperature‐controlled chamber will be useful in a wide range of biological applications where a rapid change of temperature during microscopic observations is necessary or has to be avoided allowing a simulation of ecologically relevant temperature scenarios. |
format |
Article in Journal/Newspaper |
author |
BUCHNER, O. LÜTZ, C. HOLZINGER, A. |
spellingShingle |
BUCHNER, O. LÜTZ, C. HOLZINGER, A. Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
author_facet |
BUCHNER, O. LÜTZ, C. HOLZINGER, A. |
author_sort |
BUCHNER, O. |
title |
Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
title_short |
Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
title_full |
Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
title_fullStr |
Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
title_full_unstemmed |
Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
title_sort |
design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant samples |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1365-2818.2007.01730.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2818.2007.01730.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.2007.01730.x |
genre |
Ranunculus glacialis |
genre_facet |
Ranunculus glacialis |
op_source |
Journal of Microscopy volume 225, issue 2, page 183-191 ISSN 0022-2720 1365-2818 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2818.2007.01730.x |
container_title |
Journal of Microscopy |
container_volume |
225 |
container_issue |
2 |
container_start_page |
183 |
op_container_end_page |
191 |
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1810473571138404352 |