Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection
Abstract Cardiomyopathy syndrome ( CMS ) in Atlantic salmon, S almo salar L ., is characterized by focal infiltration in the spongy myocardium and endocardium of the heart. The origin of the mononuclear infiltrate is unknown. Using experimentally infected fish, we investigated localization of the ca...
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crwiley:10.1111/j.1365-2761.2012.01431.x 2024-06-02T08:03:33+00:00 Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection Wiik‐Nielsen, J Løvoll, M Fritsvold, C Kristoffersen, A B Haugland, Ø Hordvik, I Aamelfot, M Jirillo, E Koppang, E O Grove, S 2012 http://dx.doi.org/10.1111/j.1365-2761.2012.01431.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2012.01431.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2012.01431.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 35, issue 12, page 907-916 ISSN 0140-7775 1365-2761 journal-article 2012 crwiley https://doi.org/10.1111/j.1365-2761.2012.01431.x 2024-05-03T11:06:14Z Abstract Cardiomyopathy syndrome ( CMS ) in Atlantic salmon, S almo salar L ., is characterized by focal infiltration in the spongy myocardium and endocardium of the heart. The origin of the mononuclear infiltrate is unknown. Using experimentally infected fish, we investigated localization of the causative agent, piscine myocarditis virus ( PMCV ), within the heart and characterized the cell population associated with myocardial lesions. Cellular and transcriptional characteristics in the lesions were compared with adjacent non‐infiltrated tissues using laser capture microdissection, RT ‐q PCR and immunohistochemistry. Our results reveal that PMCV is almost exclusively present in myocardial lesions. The inflammatory infiltrate comprises a variety of leucocyte populations, including T cells, B cells, MHC class II + and CD 83 + cells, most likely of the macrophage line. Correlation analyses demonstrated co‐ordinated leucocyte activity at the site of the virus infection. Cellular proliferation and/or DNA repair was demonstrated within the myocardial lesions. Different cell populations, mainly myocytes, stained positive for proliferating cell nuclear antigen ( PCNA ). Densities of endothelial cells and fibroblasts were not significantly increased. The simultaneous presence of PMCV and various inflammatory cells in all myocardial lesions analysed may indicate that both viral lytic and immunopathological effects may contribute to the pathogenesis of CMS . Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Almo ENVELOPE(15.306,15.306,66.954,66.954) Journal of Fish Diseases 35 12 907 916 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Cardiomyopathy syndrome ( CMS ) in Atlantic salmon, S almo salar L ., is characterized by focal infiltration in the spongy myocardium and endocardium of the heart. The origin of the mononuclear infiltrate is unknown. Using experimentally infected fish, we investigated localization of the causative agent, piscine myocarditis virus ( PMCV ), within the heart and characterized the cell population associated with myocardial lesions. Cellular and transcriptional characteristics in the lesions were compared with adjacent non‐infiltrated tissues using laser capture microdissection, RT ‐q PCR and immunohistochemistry. Our results reveal that PMCV is almost exclusively present in myocardial lesions. The inflammatory infiltrate comprises a variety of leucocyte populations, including T cells, B cells, MHC class II + and CD 83 + cells, most likely of the macrophage line. Correlation analyses demonstrated co‐ordinated leucocyte activity at the site of the virus infection. Cellular proliferation and/or DNA repair was demonstrated within the myocardial lesions. Different cell populations, mainly myocytes, stained positive for proliferating cell nuclear antigen ( PCNA ). Densities of endothelial cells and fibroblasts were not significantly increased. The simultaneous presence of PMCV and various inflammatory cells in all myocardial lesions analysed may indicate that both viral lytic and immunopathological effects may contribute to the pathogenesis of CMS . |
format |
Article in Journal/Newspaper |
author |
Wiik‐Nielsen, J Løvoll, M Fritsvold, C Kristoffersen, A B Haugland, Ø Hordvik, I Aamelfot, M Jirillo, E Koppang, E O Grove, S |
spellingShingle |
Wiik‐Nielsen, J Løvoll, M Fritsvold, C Kristoffersen, A B Haugland, Ø Hordvik, I Aamelfot, M Jirillo, E Koppang, E O Grove, S Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
author_facet |
Wiik‐Nielsen, J Løvoll, M Fritsvold, C Kristoffersen, A B Haugland, Ø Hordvik, I Aamelfot, M Jirillo, E Koppang, E O Grove, S |
author_sort |
Wiik‐Nielsen, J |
title |
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
title_short |
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
title_full |
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
title_fullStr |
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
title_full_unstemmed |
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection |
title_sort |
characterization of myocardial lesions associated with cardiomyopathy syndrome in atlantic salmon, salmo salar l., using laser capture microdissection |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2012.01431.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2012.01431.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2012.01431.x |
long_lat |
ENVELOPE(15.306,15.306,66.954,66.954) |
geographic |
Almo |
geographic_facet |
Almo |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 35, issue 12, page 907-916 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2012.01431.x |
container_title |
Journal of Fish Diseases |
container_volume |
35 |
container_issue |
12 |
container_start_page |
907 |
op_container_end_page |
916 |
_version_ |
1800748119451762688 |