Preliminary success using hydrogen peroxide to treat Atlantic salmon, Salmo salar L., affected with experimentally induced amoebic gill disease ( AGD)

• Published in: Journal of Fish Diseases
• Main Authors: Adams, M B, Crosbie, P B B, Nowak, B F
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2012
• Subjects: Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1365-2761.2012.01422.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2012.01422.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2012.01422.x

Abstract Currently, the only effective and commercially used treatment for amoebic gill disease ( AGD ) in farmed T asmanian A tlantic salmon is freshwater bathing. H ydrogen peroxide ( H 2 O 2 ), commonly used throughout the aquaculture industry for a range of topical skin and gill infections, was trialled in vitro and in vivo to ascertain its potential as an alternative treatment against AGD . Under in vitro conditions, trophozoites of N eoparamoeba perurans were exposed to three concentrations of H 2 O 2 in sea water (500, 1000 and 1500 mg L −1 ) over four durations (10, 20, 30 and 60 min) each at two temperatures (12 and 18 °C). Trophozoite viability was assessed immediately post‐exposure and after 24 h. A concentration/duration combination of 1000 mg L −1 for >10 min demonstrated potent amoebicidal activity. Subsequently, A tlantic salmon mildly affected with experimentally induced AGD were treated with H 2 O 2 at 12 and 18 °C for 15 min at 1250 mg L −1 and their re‐infection rate was compared to freshwater‐treated fish over 21 days. Significant differences in the percentage of filaments affected with hyperplastic lesions (in association with amoebae) and plasma osmolality were noted between treatment groups immediately post‐bath. However, the results were largely equivocal in terms of disease resolution over a 3‐week period following treatment. These data suggest that H 2 O 2 treatment in sea water successfully ameliorated a clinically light case of AGD under laboratory conditions.