Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes
Abstract This study investigated the use of alkaline hydrolysis at ambient temperature for inactivation of selected fish pathogens in fish tissues under conditions approximating those that are likely to be found in the aquaculture industry. Infectious salmon anaemia virus (ISAV) and Lactococcus garv...
Published in: | Journal of Fish Diseases |
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Language: | English |
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crwiley:10.1111/j.1365-2761.2011.01316.x 2024-10-13T14:06:07+00:00 Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes Dixon, P F Algoët, M Bayley, A Dodge, M Joiner, C Roberts, E 2011 http://dx.doi.org/10.1111/j.1365-2761.2011.01316.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2011.01316.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2011.01316.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 35, issue 1, page 65-72 ISSN 0140-7775 1365-2761 journal-article 2011 crwiley https://doi.org/10.1111/j.1365-2761.2011.01316.x 2024-09-17T04:45:31Z Abstract This study investigated the use of alkaline hydrolysis at ambient temperature for inactivation of selected fish pathogens in fish tissues under conditions approximating those that are likely to be found in the aquaculture industry. Infectious salmon anaemia virus (ISAV) and Lactococcus garvieae have been determined in a previous study to be the most resistant virus and bacteria to pH 12 from a wide range of viruses and bacteria tested. They were spiked at high titres into fish extracts that were then treated with 1 m sodium hydroxide (NaOH). Viable L. garvieae was not detected in the treated fish extract after 1 h, and ISAV was not detected after 24‐h exposure. Field mortalities of Atlantic salmon, Salmo salar L., caused by infectious pancreatic necrosis virus were treated by alkaline hydrolysis at ambient temperature. The macerated fish mortalities contained a high titre of virus (3.38 × 10 8 TCID 50 g −1 ) that was reduced to approximately 2.2 × 10 3 TCID 50 g −1 after 24‐h exposure to NaOH, and virus was not detected after exposure for 48 h. The results suggest that alkaline hydrolysis at ambient temperature has potential as a biosecure treatment method for fish by‐products containing fish pathogens. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 35 1 65 72 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract This study investigated the use of alkaline hydrolysis at ambient temperature for inactivation of selected fish pathogens in fish tissues under conditions approximating those that are likely to be found in the aquaculture industry. Infectious salmon anaemia virus (ISAV) and Lactococcus garvieae have been determined in a previous study to be the most resistant virus and bacteria to pH 12 from a wide range of viruses and bacteria tested. They were spiked at high titres into fish extracts that were then treated with 1 m sodium hydroxide (NaOH). Viable L. garvieae was not detected in the treated fish extract after 1 h, and ISAV was not detected after 24‐h exposure. Field mortalities of Atlantic salmon, Salmo salar L., caused by infectious pancreatic necrosis virus were treated by alkaline hydrolysis at ambient temperature. The macerated fish mortalities contained a high titre of virus (3.38 × 10 8 TCID 50 g −1 ) that was reduced to approximately 2.2 × 10 3 TCID 50 g −1 after 24‐h exposure to NaOH, and virus was not detected after exposure for 48 h. The results suggest that alkaline hydrolysis at ambient temperature has potential as a biosecure treatment method for fish by‐products containing fish pathogens. |
format |
Article in Journal/Newspaper |
author |
Dixon, P F Algoët, M Bayley, A Dodge, M Joiner, C Roberts, E |
spellingShingle |
Dixon, P F Algoët, M Bayley, A Dodge, M Joiner, C Roberts, E Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
author_facet |
Dixon, P F Algoët, M Bayley, A Dodge, M Joiner, C Roberts, E |
author_sort |
Dixon, P F |
title |
Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
title_short |
Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
title_full |
Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
title_fullStr |
Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
title_full_unstemmed |
Studies on the inactivation of selected viral and bacterial fish pathogens at high pH for waste disposal purposes |
title_sort |
studies on the inactivation of selected viral and bacterial fish pathogens at high ph for waste disposal purposes |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2011.01316.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2011.01316.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2011.01316.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 35, issue 1, page 65-72 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2011.01316.x |
container_title |
Journal of Fish Diseases |
container_volume |
35 |
container_issue |
1 |
container_start_page |
65 |
op_container_end_page |
72 |
_version_ |
1812812176830758912 |