Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L.
Abstract Amoebic gill disease (AGD) is a proliferative gill tissue response caused by Neoparamoeba perurans and is the main disease affecting Australian marine farmed Atlantic salmon. We have previously proposed that macroscopic gill health (‘gill score’) trajectories and challenge survival provide...
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crwiley:10.1111/j.1365-2761.2009.01108.x 2024-06-02T08:03:33+00:00 Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. Taylor, R S Crosbie, P B Cook, M T 2009 http://dx.doi.org/10.1111/j.1365-2761.2009.01108.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2009.01108.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2009.01108.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 33, issue 1, page 1-14 ISSN 0140-7775 1365-2761 journal-article 2009 crwiley https://doi.org/10.1111/j.1365-2761.2009.01108.x 2024-05-03T11:12:58Z Abstract Amoebic gill disease (AGD) is a proliferative gill tissue response caused by Neoparamoeba perurans and is the main disease affecting Australian marine farmed Atlantic salmon. We have previously proposed that macroscopic gill health (‘gill score’) trajectories and challenge survival provide evidence of a change in the nature of resistance to AGD. In order to examine whether the apparent development of resistance was because of an adaptive response, serum was sequentially sampled from the same individuals over the first three rounds of natural AGD infection and from survivors of a subsequent non‐intervention AGD survival challenge. The systemic immune reaction to ‘wildtype’ Neoparamoeba sp. was characterized by Western blot analysis and differentiated to putative carbohydrate or peptide epitopes by periodate oxidation reactions. The proportion of seropositive fish increased from 46% to 77% with each AGD round. Antibody response to carbohydrate epitope(s) was immunodominant, occurring in 43–64% of samples. Antibodies that bound peptide epitope were identified in 16% of the challenge survivors. A 1:50 (single‐dilution) enzyme‐linked immunosorbent assay confirmed a measurable immune titre in 13% of the survivors. There was no evidence that antibodies recognizing wildtype Neoparamoeba provided significant protection against AGD. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 33 1 1 14 |
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Wiley Online Library |
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English |
description |
Abstract Amoebic gill disease (AGD) is a proliferative gill tissue response caused by Neoparamoeba perurans and is the main disease affecting Australian marine farmed Atlantic salmon. We have previously proposed that macroscopic gill health (‘gill score’) trajectories and challenge survival provide evidence of a change in the nature of resistance to AGD. In order to examine whether the apparent development of resistance was because of an adaptive response, serum was sequentially sampled from the same individuals over the first three rounds of natural AGD infection and from survivors of a subsequent non‐intervention AGD survival challenge. The systemic immune reaction to ‘wildtype’ Neoparamoeba sp. was characterized by Western blot analysis and differentiated to putative carbohydrate or peptide epitopes by periodate oxidation reactions. The proportion of seropositive fish increased from 46% to 77% with each AGD round. Antibody response to carbohydrate epitope(s) was immunodominant, occurring in 43–64% of samples. Antibodies that bound peptide epitope were identified in 16% of the challenge survivors. A 1:50 (single‐dilution) enzyme‐linked immunosorbent assay confirmed a measurable immune titre in 13% of the survivors. There was no evidence that antibodies recognizing wildtype Neoparamoeba provided significant protection against AGD. |
format |
Article in Journal/Newspaper |
author |
Taylor, R S Crosbie, P B Cook, M T |
spellingShingle |
Taylor, R S Crosbie, P B Cook, M T Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
author_facet |
Taylor, R S Crosbie, P B Cook, M T |
author_sort |
Taylor, R S |
title |
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
title_short |
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
title_full |
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
title_fullStr |
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L. |
title_sort |
amoebic gill disease resistance is not related to the systemic antibody response of atlantic salmon, salmo salar l. |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2009.01108.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2009.01108.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2009.01108.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 33, issue 1, page 1-14 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2009.01108.x |
container_title |
Journal of Fish Diseases |
container_volume |
33 |
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1 |
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1 |
op_container_end_page |
14 |
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1800748097613070336 |