Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence
Abstract Prospective longitudinal studies of two outbreaks of pancreas disease in Atlantic salmon (AS), Salmo salar L., in Ireland were conducted. Both outbreaks occurred during the marine phase of production, with one caused by salmonid alphavirus subtype 1 (SAV1) and the other by SAV4. In addition...
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crwiley:10.1111/j.1365-2761.2009.01096.x 2024-09-09T19:30:30+00:00 Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence Graham, D A Fringuelli, E Wilson, C Rowley, H M Brown, A Rodger, H McLoughlin, M F McManus, C Casey, E McCarthy, L J Ruane, N M 2010 http://dx.doi.org/10.1111/j.1365-2761.2009.01096.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2009.01096.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2009.01096.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 33, issue 2, page 123-135 ISSN 0140-7775 1365-2761 journal-article 2010 crwiley https://doi.org/10.1111/j.1365-2761.2009.01096.x 2024-07-23T04:11:17Z Abstract Prospective longitudinal studies of two outbreaks of pancreas disease in Atlantic salmon (AS), Salmo salar L., in Ireland were conducted. Both outbreaks occurred during the marine phase of production, with one caused by salmonid alphavirus subtype 1 (SAV1) and the other by SAV4. In addition to screening a range of tissues by real‐time reverse transcriptase polymerase chain reaction (RRT‐PCR), virological, serological and histopathological examinations were performed along with partial genome sequencing and results were related to environmental and production data and farm history. On Farm 1 (marine sampling only), infection was detected within 3 weeks of smolts being placed on the farm, while on Farm 2 (freshwater and marine sampling), infection was first detected 315 days after transfer to sea. In both outbreaks, RRT‐PCR signals were detected in a range of tissues including gill, heart, kidney, pancreas/pyloric caeca, brain and serum. Persistence of signal was longest in gill and heart (≥265 days on both farms) and shortest in serum. Mortalities on the two farms varied from 10.9% to 30%. In both cases, partial genome sequence of the causative viruses were identical to SAV strains detected in previous populations of AS on each of the study farms, including populations with which the study populations overlapped in time and space. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Fish Diseases 33 2 123 135 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Prospective longitudinal studies of two outbreaks of pancreas disease in Atlantic salmon (AS), Salmo salar L., in Ireland were conducted. Both outbreaks occurred during the marine phase of production, with one caused by salmonid alphavirus subtype 1 (SAV1) and the other by SAV4. In addition to screening a range of tissues by real‐time reverse transcriptase polymerase chain reaction (RRT‐PCR), virological, serological and histopathological examinations were performed along with partial genome sequencing and results were related to environmental and production data and farm history. On Farm 1 (marine sampling only), infection was detected within 3 weeks of smolts being placed on the farm, while on Farm 2 (freshwater and marine sampling), infection was first detected 315 days after transfer to sea. In both outbreaks, RRT‐PCR signals were detected in a range of tissues including gill, heart, kidney, pancreas/pyloric caeca, brain and serum. Persistence of signal was longest in gill and heart (≥265 days on both farms) and shortest in serum. Mortalities on the two farms varied from 10.9% to 30%. In both cases, partial genome sequence of the causative viruses were identical to SAV strains detected in previous populations of AS on each of the study farms, including populations with which the study populations overlapped in time and space. |
format |
Article in Journal/Newspaper |
author |
Graham, D A Fringuelli, E Wilson, C Rowley, H M Brown, A Rodger, H McLoughlin, M F McManus, C Casey, E McCarthy, L J Ruane, N M |
spellingShingle |
Graham, D A Fringuelli, E Wilson, C Rowley, H M Brown, A Rodger, H McLoughlin, M F McManus, C Casey, E McCarthy, L J Ruane, N M Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
author_facet |
Graham, D A Fringuelli, E Wilson, C Rowley, H M Brown, A Rodger, H McLoughlin, M F McManus, C Casey, E McCarthy, L J Ruane, N M |
author_sort |
Graham, D A |
title |
Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
title_short |
Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
title_full |
Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
title_fullStr |
Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
title_full_unstemmed |
Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistence |
title_sort |
prospective longitudinal studies of salmonid alphavirus infections on two atlantic salmon farms in ireland; evidence for viral persistence |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2009.01096.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2009.01096.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2009.01096.x |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 33, issue 2, page 123-135 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2009.01096.x |
container_title |
Journal of Fish Diseases |
container_volume |
33 |
container_issue |
2 |
container_start_page |
123 |
op_container_end_page |
135 |
_version_ |
1809899477517991936 |