Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease
Abstract The recent description of Neoparamoeba perurans as an aetiological agent of amoebic gill disease (AGD) advanced our understanding of the condition and has forced a re‐evaluation of methods used for the diagnosis of AGD. Currently, there are no tools available that are both specific for N. p...
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crwiley:10.1111/j.1365-2761.2008.00903.x 2024-06-02T08:03:39+00:00 Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease Young, N D Dyková, I Nowak, B F Morrison, R N 2008 http://dx.doi.org/10.1111/j.1365-2761.2008.00903.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2008.00903.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2008.00903.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 31, issue 4, page 285-295 ISSN 0140-7775 1365-2761 journal-article 2008 crwiley https://doi.org/10.1111/j.1365-2761.2008.00903.x 2024-05-03T11:19:17Z Abstract The recent description of Neoparamoeba perurans as an aetiological agent of amoebic gill disease (AGD) advanced our understanding of the condition and has forced a re‐evaluation of methods used for the diagnosis of AGD. Currently, there are no tools available that are both specific for N. perurans and suitable for a routine diagnostic procedure. Therefore, in this study we describe an assay to detect N. perurans . The assay, which utilizes PCR to amplify the N. perurans 18S rRNA gene, was shown to be specific and highly sensitive. Neoparamoeba perurans was detected in both gill samples and primary isolates of non‐cultured gill‐derived amoebae obtained during necropsy or biopsy from AGD‐affected Atlantic salmon, Salmo salar L. The PCR‐based assay provides a simple, flexible tool that will be a useful addition to the diagnostic repertoire for AGD. It may also be used for the genotypic screening of trophozoites during culture and could facilitate further epidemiological and ecological studies of AGD. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 31 4 285 295 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The recent description of Neoparamoeba perurans as an aetiological agent of amoebic gill disease (AGD) advanced our understanding of the condition and has forced a re‐evaluation of methods used for the diagnosis of AGD. Currently, there are no tools available that are both specific for N. perurans and suitable for a routine diagnostic procedure. Therefore, in this study we describe an assay to detect N. perurans . The assay, which utilizes PCR to amplify the N. perurans 18S rRNA gene, was shown to be specific and highly sensitive. Neoparamoeba perurans was detected in both gill samples and primary isolates of non‐cultured gill‐derived amoebae obtained during necropsy or biopsy from AGD‐affected Atlantic salmon, Salmo salar L. The PCR‐based assay provides a simple, flexible tool that will be a useful addition to the diagnostic repertoire for AGD. It may also be used for the genotypic screening of trophozoites during culture and could facilitate further epidemiological and ecological studies of AGD. |
format |
Article in Journal/Newspaper |
author |
Young, N D Dyková, I Nowak, B F Morrison, R N |
spellingShingle |
Young, N D Dyková, I Nowak, B F Morrison, R N Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
author_facet |
Young, N D Dyková, I Nowak, B F Morrison, R N |
author_sort |
Young, N D |
title |
Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
title_short |
Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
title_full |
Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
title_fullStr |
Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
title_full_unstemmed |
Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease |
title_sort |
development of a diagnostic pcr to detect neoparamoeba perurans, agent of amoebic gill disease |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2008.00903.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2008.00903.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2008.00903.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 31, issue 4, page 285-295 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2008.00903.x |
container_title |
Journal of Fish Diseases |
container_volume |
31 |
container_issue |
4 |
container_start_page |
285 |
op_container_end_page |
295 |
_version_ |
1800748242878595072 |