Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L.
Abstract Methods for the isolation and quantification of infectious pancreatic necrosis virus (IPNV) from ovarian and seminal fluids of Atlantic salmon are described. Both have utility for the non‐lethal detection of IPNV in mature broodstock and for research into vertical transmission. Two experime...
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crwiley:10.1111/j.1365-2761.2007.00866.x 2024-09-15T17:55:51+00:00 Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. Smail, D A Munro, E S 2007 http://dx.doi.org/10.1111/j.1365-2761.2007.00866.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2007.00866.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2007.00866.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 31, issue 1, page 49-58 ISSN 0140-7775 1365-2761 journal-article 2007 crwiley https://doi.org/10.1111/j.1365-2761.2007.00866.x 2024-07-23T04:12:24Z Abstract Methods for the isolation and quantification of infectious pancreatic necrosis virus (IPNV) from ovarian and seminal fluids of Atlantic salmon are described. Both have utility for the non‐lethal detection of IPNV in mature broodstock and for research into vertical transmission. Two experiments are described to check the efficiency of an elution method for the removal of IPNV from milt. The isolation rate for ovarian fluid of females was generally higher than that for seminal fluid of males from the same populations. In IPNV milt mixing experiments up to 99.98% of available IPNV adsorbed to Atlantic salmon spermatozoa and 20–100% of virus eluted using a variety of procedures. Titration of virus from naturally infected milt can be useful in estimating the relative vertical transmission risk from male broodstock. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 31 1 49 58 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Methods for the isolation and quantification of infectious pancreatic necrosis virus (IPNV) from ovarian and seminal fluids of Atlantic salmon are described. Both have utility for the non‐lethal detection of IPNV in mature broodstock and for research into vertical transmission. Two experiments are described to check the efficiency of an elution method for the removal of IPNV from milt. The isolation rate for ovarian fluid of females was generally higher than that for seminal fluid of males from the same populations. In IPNV milt mixing experiments up to 99.98% of available IPNV adsorbed to Atlantic salmon spermatozoa and 20–100% of virus eluted using a variety of procedures. Titration of virus from naturally infected milt can be useful in estimating the relative vertical transmission risk from male broodstock. |
format |
Article in Journal/Newspaper |
author |
Smail, D A Munro, E S |
spellingShingle |
Smail, D A Munro, E S Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
author_facet |
Smail, D A Munro, E S |
author_sort |
Smail, D A |
title |
Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
title_short |
Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
title_full |
Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
title_fullStr |
Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L. |
title_sort |
isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of atlantic salmon, salmo salar l. |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2007.00866.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2007.00866.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2007.00866.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 31, issue 1, page 49-58 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2007.00866.x |
container_title |
Journal of Fish Diseases |
container_volume |
31 |
container_issue |
1 |
container_start_page |
49 |
op_container_end_page |
58 |
_version_ |
1810432078545682432 |