Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L.
Abstract Juvenile Atlantic cod (10 g) were infected with infectious pancreatic necrosis virus (IPNV) by intraperitoneal injection and cohabitation. Fish showed no signs of disease but IPNV could be re‐isolated from kidney tissue for up to 12 weeks. On weeks 2, 5, 8, 10, 11 and 12 following infection...
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crwiley:10.1111/j.1365-2761.2006.00731.x 2024-06-02T08:03:09+00:00 Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. Garcia, J Urquhart, K Ellis, A E 2006 http://dx.doi.org/10.1111/j.1365-2761.2006.00731.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2006.00731.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2006.00731.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 29, issue 7, page 409-413 ISSN 0140-7775 1365-2761 journal-article 2006 crwiley https://doi.org/10.1111/j.1365-2761.2006.00731.x 2024-05-03T10:50:22Z Abstract Juvenile Atlantic cod (10 g) were infected with infectious pancreatic necrosis virus (IPNV) by intraperitoneal injection and cohabitation. Fish showed no signs of disease but IPNV could be re‐isolated from kidney tissue for up to 12 weeks. On weeks 2, 5, 8, 10, 11 and 12 following infection, kidney leucocytes were fractionated on Percoll gradients, and cells separated into plastic adherent and non‐adherent cell populations after overnight incubation. IPNV was detectable in lysates of both cell populations and in supernatants by culture in CHSE‐214 cells. Wells containing 10 5 −10 6 macrophages had an IPNV TCID 50 of about 10 3 /well and in serially diluted macrophages the minimum number of cells required to detect virus ranged from 10 1 to 10 4 . These data indicate that about one in 10 4 macrophages were infected and the mean number of virus/infected cell was about 10. Replication of IPNV in the macrophages was low as the titre of the virus in macrophage lysates did not increase between days 1 and 3 of culturing the macrophages, but virus was released into the supernatant over this time. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Journal of Fish Diseases 29 7 409 413 |
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Wiley Online Library |
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English |
description |
Abstract Juvenile Atlantic cod (10 g) were infected with infectious pancreatic necrosis virus (IPNV) by intraperitoneal injection and cohabitation. Fish showed no signs of disease but IPNV could be re‐isolated from kidney tissue for up to 12 weeks. On weeks 2, 5, 8, 10, 11 and 12 following infection, kidney leucocytes were fractionated on Percoll gradients, and cells separated into plastic adherent and non‐adherent cell populations after overnight incubation. IPNV was detectable in lysates of both cell populations and in supernatants by culture in CHSE‐214 cells. Wells containing 10 5 −10 6 macrophages had an IPNV TCID 50 of about 10 3 /well and in serially diluted macrophages the minimum number of cells required to detect virus ranged from 10 1 to 10 4 . These data indicate that about one in 10 4 macrophages were infected and the mean number of virus/infected cell was about 10. Replication of IPNV in the macrophages was low as the titre of the virus in macrophage lysates did not increase between days 1 and 3 of culturing the macrophages, but virus was released into the supernatant over this time. |
format |
Article in Journal/Newspaper |
author |
Garcia, J Urquhart, K Ellis, A E |
spellingShingle |
Garcia, J Urquhart, K Ellis, A E Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
author_facet |
Garcia, J Urquhart, K Ellis, A E |
author_sort |
Garcia, J |
title |
Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
title_short |
Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
title_full |
Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
title_fullStr |
Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
title_full_unstemmed |
Infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile Atlantic cod, Gadus morhua L. |
title_sort |
infectious pancreatic necrosis virus establishes an asymptomatic carrier state in kidney leucocytes of juvenile atlantic cod, gadus morhua l. |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2006.00731.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2006.00731.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2006.00731.x |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of Fish Diseases volume 29, issue 7, page 409-413 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2006.00731.x |
container_title |
Journal of Fish Diseases |
container_volume |
29 |
container_issue |
7 |
container_start_page |
409 |
op_container_end_page |
413 |
_version_ |
1800747623944028160 |