Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.)
Abstract A non‐destructive procedure was utilized to determine the infectious pancreatic necrosis virus (IPNV) status of an apparently healthy turbot broodstock. Blood samples were used to detect IPNV by reverse transcriptase‐polymerase chain reaction (RT‐PCR), Southern blot hybridization and nested...
Published in: | Journal of Fish Diseases |
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Online Access: | http://dx.doi.org/10.1111/j.1365-2761.2005.00675.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00675.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00675.x |
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crwiley:10.1111/j.1365-2761.2005.00675.x 2024-06-02T08:14:11+00:00 Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) Cutrín, J M López‐Vázquez, C Olveira, J G Castro, S Dopazo, C P Bandín, I 2005 http://dx.doi.org/10.1111/j.1365-2761.2005.00675.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00675.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00675.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 28, issue 12, page 713-722 ISSN 0140-7775 1365-2761 journal-article 2005 crwiley https://doi.org/10.1111/j.1365-2761.2005.00675.x 2024-05-03T12:06:22Z Abstract A non‐destructive procedure was utilized to determine the infectious pancreatic necrosis virus (IPNV) status of an apparently healthy turbot broodstock. Blood samples were used to detect IPNV by reverse transcriptase‐polymerase chain reaction (RT‐PCR), Southern blot hybridization and nested PCR. In addition, viral isolation from turbot leucocytes was performed. Around 22% of the fish were IPNV positive by RT‐PCR, and this increased to close to 60% when nested PCR was performed. The present report supports the use of blood samples for the detection of IPNV‐like viruses in brood fish. In addition, we demonstrate that it is possible to isolate the virus from the blood of carrier fish, as a non‐lethal detection method, although it is much less sensitive than RT‐PCR and nested PCR as a IPNV‐like strain was isolated from only five of the 15 blood sample pools assayed. The viral isolate was identified as type Dry Mills (genogroup I) by means of restriction fragment length polymorphisms and DNA sequencing. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of Fish Diseases 28 12 713 722 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A non‐destructive procedure was utilized to determine the infectious pancreatic necrosis virus (IPNV) status of an apparently healthy turbot broodstock. Blood samples were used to detect IPNV by reverse transcriptase‐polymerase chain reaction (RT‐PCR), Southern blot hybridization and nested PCR. In addition, viral isolation from turbot leucocytes was performed. Around 22% of the fish were IPNV positive by RT‐PCR, and this increased to close to 60% when nested PCR was performed. The present report supports the use of blood samples for the detection of IPNV‐like viruses in brood fish. In addition, we demonstrate that it is possible to isolate the virus from the blood of carrier fish, as a non‐lethal detection method, although it is much less sensitive than RT‐PCR and nested PCR as a IPNV‐like strain was isolated from only five of the 15 blood sample pools assayed. The viral isolate was identified as type Dry Mills (genogroup I) by means of restriction fragment length polymorphisms and DNA sequencing. |
format |
Article in Journal/Newspaper |
author |
Cutrín, J M López‐Vázquez, C Olveira, J G Castro, S Dopazo, C P Bandín, I |
spellingShingle |
Cutrín, J M López‐Vázquez, C Olveira, J G Castro, S Dopazo, C P Bandín, I Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
author_facet |
Cutrín, J M López‐Vázquez, C Olveira, J G Castro, S Dopazo, C P Bandín, I |
author_sort |
Cutrín, J M |
title |
Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
title_short |
Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
title_full |
Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
title_fullStr |
Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
title_full_unstemmed |
Isolation in cell culture and detection by PCR‐based technology of IPNV‐like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.) |
title_sort |
isolation in cell culture and detection by pcr‐based technology of ipnv‐like virus from leucocytes of carrier turbot, scophthalmus maximus (l.) |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2005.00675.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00675.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00675.x |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Fish Diseases volume 28, issue 12, page 713-722 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2005.00675.x |
container_title |
Journal of Fish Diseases |
container_volume |
28 |
container_issue |
12 |
container_start_page |
713 |
op_container_end_page |
722 |
_version_ |
1800737905164943360 |