Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard
Abstract Reverse transcriptase‐polymerase chain reaction (RT‐PCR), virus isolation (VI) and indirect fluorescent antibody test (IFAT) are three tests currently used by the salmon industry to identify fish infected with the infectious salmon anaemia virus (ISAV). However, very limited information is...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2005
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1365-2761.2005.00612.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00612.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00612.x |
id |
crwiley:10.1111/j.1365-2761.2005.00612.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1365-2761.2005.00612.x 2024-06-02T08:03:42+00:00 Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard Nérette, P Dohoo, I Hammell, L 2005 http://dx.doi.org/10.1111/j.1365-2761.2005.00612.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00612.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00612.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 28, issue 2, page 89-99 ISSN 0140-7775 1365-2761 journal-article 2005 crwiley https://doi.org/10.1111/j.1365-2761.2005.00612.x 2024-05-03T12:07:35Z Abstract Reverse transcriptase‐polymerase chain reaction (RT‐PCR), virus isolation (VI) and indirect fluorescent antibody test (IFAT) are three tests currently used by the salmon industry to identify fish infected with the infectious salmon anaemia virus (ISAV). However, very limited information is available on the sensitivity and specificity of these methods. In order to evaluate these tests in fish representing a range of farmed Atlantic salmon populations, five laboratories participated in a blind study of 400 kidney samples from four groups of fish with different prevalences of ISAV. Each laboratory used its own testing protocols. Estimates of the specificity of each test were determined directly from a population assumed to be free of infection. Indirect estimates of the sensitivity and specificity of each test were obtained using maximum likelihood estimation of a latent class model (i.e. no gold standard test result available). There was a substantial difference in sensitivity and specificity of RT‐PCR among the three laboratories using this test. If only the best results for the RT‐PCR tests are taken into account, the maximum likelihood estimates obtained from this study suggest RT‐PCR and VI are of similar high sensitivity (range 92–100%), IFAT is the least sensitive method (range 65–76%) while the three tests have similar high specificities (range 96–100%). The results of the study suggest: (1) RT‐PCR tests should be standardized before they are used as a diagnostic test for prevention and control of ISAV, (2) the sensitivity of VI was higher than expected and (3) IFAT has a low sensitivity but might be a good screening test because of its low cost. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Fish Diseases 28 2 89 99 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Reverse transcriptase‐polymerase chain reaction (RT‐PCR), virus isolation (VI) and indirect fluorescent antibody test (IFAT) are three tests currently used by the salmon industry to identify fish infected with the infectious salmon anaemia virus (ISAV). However, very limited information is available on the sensitivity and specificity of these methods. In order to evaluate these tests in fish representing a range of farmed Atlantic salmon populations, five laboratories participated in a blind study of 400 kidney samples from four groups of fish with different prevalences of ISAV. Each laboratory used its own testing protocols. Estimates of the specificity of each test were determined directly from a population assumed to be free of infection. Indirect estimates of the sensitivity and specificity of each test were obtained using maximum likelihood estimation of a latent class model (i.e. no gold standard test result available). There was a substantial difference in sensitivity and specificity of RT‐PCR among the three laboratories using this test. If only the best results for the RT‐PCR tests are taken into account, the maximum likelihood estimates obtained from this study suggest RT‐PCR and VI are of similar high sensitivity (range 92–100%), IFAT is the least sensitive method (range 65–76%) while the three tests have similar high specificities (range 96–100%). The results of the study suggest: (1) RT‐PCR tests should be standardized before they are used as a diagnostic test for prevention and control of ISAV, (2) the sensitivity of VI was higher than expected and (3) IFAT has a low sensitivity but might be a good screening test because of its low cost. |
format |
Article in Journal/Newspaper |
author |
Nérette, P Dohoo, I Hammell, L |
spellingShingle |
Nérette, P Dohoo, I Hammell, L Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
author_facet |
Nérette, P Dohoo, I Hammell, L |
author_sort |
Nérette, P |
title |
Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
title_short |
Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
title_full |
Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
title_fullStr |
Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
title_full_unstemmed |
Estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
title_sort |
estimation of specificity and sensitivity of three diagnostic tests for infectious salmon anaemia virus in the absence of a gold standard |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2005.00612.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2005.00612.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2005.00612.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases volume 28, issue 2, page 89-99 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2005.00612.x |
container_title |
Journal of Fish Diseases |
container_volume |
28 |
container_issue |
2 |
container_start_page |
89 |
op_container_end_page |
99 |
_version_ |
1800748312814419968 |