Nodavirus infections in Israeli mariculture
Abstract Viral encephalopathy and retinopathy (VER) infections were diagnosed in five fish species: Epinephelus aeneus , Dicentrarchus labrax , Sciaenops ocellatus , Lates calcarifer and Mugil cephalus cultured on both the Red Sea and Mediterranean coasts of Israel during 1998–2002. Spongiform vacuo...
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crwiley:10.1111/j.1365-2761.2004.00565.x 2024-06-02T08:14:09+00:00 Nodavirus infections in Israeli mariculture Ucko, M Colorni, A Diamant, A 2004 http://dx.doi.org/10.1111/j.1365-2761.2004.00565.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2004.00565.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2004.00565.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 27, issue 8, page 459-469 ISSN 0140-7775 1365-2761 journal-article 2004 crwiley https://doi.org/10.1111/j.1365-2761.2004.00565.x 2024-05-03T11:39:26Z Abstract Viral encephalopathy and retinopathy (VER) infections were diagnosed in five fish species: Epinephelus aeneus , Dicentrarchus labrax , Sciaenops ocellatus , Lates calcarifer and Mugil cephalus cultured on both the Red Sea and Mediterranean coasts of Israel during 1998–2002. Spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. With transmission electron microscopy, paracrystalline arrays and pieces of membrane‐associated non‐enveloped virions measuring approximately 30 nm in diameter were observed in the brain and retina of all species. At the molecular level, the nodavirus was detected by using a primer set that amplified the T4 region of the coat protein gene. When the same set of primers was used to search for VER in an additional fish species, Sparus aurata , it was found to produce non‐specific amplicons, giving rise to false‐positive results. This problem was overcome by using a different primer set (F1/VR3), designed on a highly conserved region of the virus gene, which amplified a fragment of 254 bp, and confirmed that S. aurata was nodavirus‐free. This set was validated on all five species of infected fish, as well as clinically healthy fish. Comparison of the coat protein genes from the Israeli isolated sequences indicated that more than one viral strain was involved. No strict host‐specificity was evident. Red Sea and Mediterranean isolated sequences grouped in distinct clusters, together with several foreign isolates from the Mediterranean area and the Far East, as phylogenetically close to the Epinephelus akaara RGNNV type. Article in Journal/Newspaper Sciaenops ocellatus Wiley Online Library Journal of Fish Diseases 27 8 459 469 |
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Abstract Viral encephalopathy and retinopathy (VER) infections were diagnosed in five fish species: Epinephelus aeneus , Dicentrarchus labrax , Sciaenops ocellatus , Lates calcarifer and Mugil cephalus cultured on both the Red Sea and Mediterranean coasts of Israel during 1998–2002. Spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. With transmission electron microscopy, paracrystalline arrays and pieces of membrane‐associated non‐enveloped virions measuring approximately 30 nm in diameter were observed in the brain and retina of all species. At the molecular level, the nodavirus was detected by using a primer set that amplified the T4 region of the coat protein gene. When the same set of primers was used to search for VER in an additional fish species, Sparus aurata , it was found to produce non‐specific amplicons, giving rise to false‐positive results. This problem was overcome by using a different primer set (F1/VR3), designed on a highly conserved region of the virus gene, which amplified a fragment of 254 bp, and confirmed that S. aurata was nodavirus‐free. This set was validated on all five species of infected fish, as well as clinically healthy fish. Comparison of the coat protein genes from the Israeli isolated sequences indicated that more than one viral strain was involved. No strict host‐specificity was evident. Red Sea and Mediterranean isolated sequences grouped in distinct clusters, together with several foreign isolates from the Mediterranean area and the Far East, as phylogenetically close to the Epinephelus akaara RGNNV type. |
format |
Article in Journal/Newspaper |
author |
Ucko, M Colorni, A Diamant, A |
spellingShingle |
Ucko, M Colorni, A Diamant, A Nodavirus infections in Israeli mariculture |
author_facet |
Ucko, M Colorni, A Diamant, A |
author_sort |
Ucko, M |
title |
Nodavirus infections in Israeli mariculture |
title_short |
Nodavirus infections in Israeli mariculture |
title_full |
Nodavirus infections in Israeli mariculture |
title_fullStr |
Nodavirus infections in Israeli mariculture |
title_full_unstemmed |
Nodavirus infections in Israeli mariculture |
title_sort |
nodavirus infections in israeli mariculture |
publisher |
Wiley |
publishDate |
2004 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2004.00565.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2004.00565.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2004.00565.x |
genre |
Sciaenops ocellatus |
genre_facet |
Sciaenops ocellatus |
op_source |
Journal of Fish Diseases volume 27, issue 8, page 459-469 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2004.00565.x |
container_title |
Journal of Fish Diseases |
container_volume |
27 |
container_issue |
8 |
container_start_page |
459 |
op_container_end_page |
469 |
_version_ |
1800737871930327040 |