Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum)
Abstract A simple method of detecting the presence of the salmonid alphaviruses (SAVs), salmon pancreas disease virus (SPDV) and sleeping disease virus (SDV), from serum samples is described. Using a 96‐well tissue‐culture plate format, test sera are diluted in medium and added to chinook salmon emb...
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crwiley:10.1111/j.1365-2761.2004.00530.x 2024-09-30T14:32:31+00:00 Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) Jewhurst, V A Todd, D Rowley, H M Walker, I W Weston, J H McLoughlin, M F Graham, D A 2004 http://dx.doi.org/10.1111/j.1365-2761.2004.00530.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2004.00530.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2004.00530.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 27, issue 3, page 143-149 ISSN 0140-7775 1365-2761 journal-article 2004 crwiley https://doi.org/10.1111/j.1365-2761.2004.00530.x 2024-09-03T04:23:49Z Abstract A simple method of detecting the presence of the salmonid alphaviruses (SAVs), salmon pancreas disease virus (SPDV) and sleeping disease virus (SDV), from serum samples is described. Using a 96‐well tissue‐culture plate format, test sera are diluted in medium and added to chinook salmon embryo (CHSE‐214) cells. After incubation for 3 days at 15 °C, plates are fixed and stained using a monoclonal antibody (mAb)‐based immunoperoxidase (IPX) detection system, and virus‐infected cells are observed microscopically by white light. Application of this screening test, which is now used routinely in our laboratory in conjunction with an IPX‐based virus neutralization (IPX‐VN) test for detecting antibodies to SAVs, has resulted in the recovery of 12 additional isolates from salmon sera and four additional isolates from trout sera. A low level of antigenic variation was detected when these SAV isolates were investigated by indirect immunofluorescence using a panel of mAbs raised to reference SPDV and SDV isolates. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Fish Diseases 27 3 143 149 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract A simple method of detecting the presence of the salmonid alphaviruses (SAVs), salmon pancreas disease virus (SPDV) and sleeping disease virus (SDV), from serum samples is described. Using a 96‐well tissue‐culture plate format, test sera are diluted in medium and added to chinook salmon embryo (CHSE‐214) cells. After incubation for 3 days at 15 °C, plates are fixed and stained using a monoclonal antibody (mAb)‐based immunoperoxidase (IPX) detection system, and virus‐infected cells are observed microscopically by white light. Application of this screening test, which is now used routinely in our laboratory in conjunction with an IPX‐based virus neutralization (IPX‐VN) test for detecting antibodies to SAVs, has resulted in the recovery of 12 additional isolates from salmon sera and four additional isolates from trout sera. A low level of antigenic variation was detected when these SAV isolates were investigated by indirect immunofluorescence using a panel of mAbs raised to reference SPDV and SDV isolates. |
format |
Article in Journal/Newspaper |
author |
Jewhurst, V A Todd, D Rowley, H M Walker, I W Weston, J H McLoughlin, M F Graham, D A |
spellingShingle |
Jewhurst, V A Todd, D Rowley, H M Walker, I W Weston, J H McLoughlin, M F Graham, D A Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
author_facet |
Jewhurst, V A Todd, D Rowley, H M Walker, I W Weston, J H McLoughlin, M F Graham, D A |
author_sort |
Jewhurst, V A |
title |
Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
title_short |
Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
title_full |
Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
title_fullStr |
Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
title_full_unstemmed |
Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
title_sort |
detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed atlantic salmon, salmo salar l., and farmed rainbow trout, oncorhynchus mykiss (walbaum) |
publisher |
Wiley |
publishDate |
2004 |
url |
http://dx.doi.org/10.1111/j.1365-2761.2004.00530.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2004.00530.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2004.00530.x |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 27, issue 3, page 143-149 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.2004.00530.x |
container_title |
Journal of Fish Diseases |
container_volume |
27 |
container_issue |
3 |
container_start_page |
143 |
op_container_end_page |
149 |
_version_ |
1811636662391799808 |