Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum?
Abstract The role of the intestinal tract in Vibrio anguillarum infection of turbot, Scophthalmus maximus (L.), fingerlings was investigated in two in vivo models and the possible mechanisms involved were studied in vitro. Viable V. anguillarum cells were detected in spleens from more than 50% of th...
Published in: | Journal of Fish Diseases |
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Language: | English |
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Wiley
1996
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Online Access: | http://dx.doi.org/10.1111/j.1365-2761.1996.tb00129.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00129.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00129.x |
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crwiley:10.1111/j.1365-2761.1996.tb00129.x 2024-06-02T08:14:10+00:00 Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? Oisson, J C Jöborn, A Westerdahl, A Blomberg, L Kjelleberg, S Conway, P L 1996 http://dx.doi.org/10.1111/j.1365-2761.1996.tb00129.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00129.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00129.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 19, issue 3, page 225-234 ISSN 0140-7775 1365-2761 journal-article 1996 crwiley https://doi.org/10.1111/j.1365-2761.1996.tb00129.x 2024-05-03T11:49:11Z Abstract The role of the intestinal tract in Vibrio anguillarum infection of turbot, Scophthalmus maximus (L.), fingerlings was investigated in two in vivo models and the possible mechanisms involved were studied in vitro. Viable V. anguillarum cells were detected in spleens from more than 50% of the fish administered the pathogen orally or rectally, suggesting that the intestinal tract is a portal of entry for V anguillarum. In transmission electron micrographs, V. anguillarum ‐ like cells were seen close to the rectal epithelium, suggesting penetration of the mucus layer, but no epithelial cell penetration or endocytosis was evident. Attachment to intact turbot intestines was investigated, and 80% or more of the bacterial cells still remained attached after serial washings. A significantly higher number of cells attached to rectal segments than to the other intestinal segments. In vitro, V. anguillarum cells did not adhere specifically to intestinal mucus, but rather accumulated close to intestinal mucus interfaces and subsequently penetrated them. It is proposed that the intestinal ttact of tutbot is a portal of entry for V. anguillarum and that the cells penetrate the intestinal mucus overlaying the epithelial cells. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of Fish Diseases 19 3 225 234 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The role of the intestinal tract in Vibrio anguillarum infection of turbot, Scophthalmus maximus (L.), fingerlings was investigated in two in vivo models and the possible mechanisms involved were studied in vitro. Viable V. anguillarum cells were detected in spleens from more than 50% of the fish administered the pathogen orally or rectally, suggesting that the intestinal tract is a portal of entry for V anguillarum. In transmission electron micrographs, V. anguillarum ‐ like cells were seen close to the rectal epithelium, suggesting penetration of the mucus layer, but no epithelial cell penetration or endocytosis was evident. Attachment to intact turbot intestines was investigated, and 80% or more of the bacterial cells still remained attached after serial washings. A significantly higher number of cells attached to rectal segments than to the other intestinal segments. In vitro, V. anguillarum cells did not adhere specifically to intestinal mucus, but rather accumulated close to intestinal mucus interfaces and subsequently penetrated them. It is proposed that the intestinal ttact of tutbot is a portal of entry for V. anguillarum and that the cells penetrate the intestinal mucus overlaying the epithelial cells. |
format |
Article in Journal/Newspaper |
author |
Oisson, J C Jöborn, A Westerdahl, A Blomberg, L Kjelleberg, S Conway, P L |
spellingShingle |
Oisson, J C Jöborn, A Westerdahl, A Blomberg, L Kjelleberg, S Conway, P L Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
author_facet |
Oisson, J C Jöborn, A Westerdahl, A Blomberg, L Kjelleberg, S Conway, P L |
author_sort |
Oisson, J C |
title |
Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
title_short |
Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
title_full |
Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
title_fullStr |
Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
title_full_unstemmed |
Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? |
title_sort |
is the turbot, scophthalmus maximus (l.), intestine a portal of entry for the fish pathogen vibrio anguillarum? |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1996.tb00129.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00129.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00129.x |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Fish Diseases volume 19, issue 3, page 225-234 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1996.tb00129.x |
container_title |
Journal of Fish Diseases |
container_volume |
19 |
container_issue |
3 |
container_start_page |
225 |
op_container_end_page |
234 |
_version_ |
1800737891405529088 |