Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques
Abstract The results of comparative immunohisto‐chemical staining studies with two lectin probes, GS‐I and SBA, and five monoclonal antibodies raised against the PKX myxosporean are presented. In order to investigate the postulated link between PKX and Sphaerospora spp., the lectins and monoclonal a...
Published in: | Journal of Fish Diseases |
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1996
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Online Access: | http://dx.doi.org/10.1111/j.1365-2761.1996.tb00120.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00120.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00120.x |
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crwiley:10.1111/j.1365-2761.1996.tb00120.x 2024-06-02T08:03:40+00:00 Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques de Mateo, M Marin McGeorge, J Morris, D Kent, M L 1996 http://dx.doi.org/10.1111/j.1365-2761.1996.tb00120.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00120.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00120.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 19, issue 1, page 55-63 ISSN 0140-7775 1365-2761 journal-article 1996 crwiley https://doi.org/10.1111/j.1365-2761.1996.tb00120.x 2024-05-03T12:06:06Z Abstract The results of comparative immunohisto‐chemical staining studies with two lectin probes, GS‐I and SBA, and five monoclonal antibodies raised against the PKX myxosporean are presented. In order to investigate the postulated link between PKX and Sphaerospora spp., the lectins and monoclonal antibodies were tested against PKX, Sphaerospora renicola, Sphaerospora oncorhynchi, Sphaerospora elegam, Sphaerospora truttae and Sphaero‐spora sp. from Atlantic salmon, Salmo salar L., and Ceratomyxa shasta. SBA appears a useful tool for tbe diagnosis of all Sphaerospora spp. infections and was also positive for C. shasta . GS‐I and monoclonal antibodies (12, C5, D4 and A3) appear to be specific for both the extrasporogonic and sporogonic stages of PKX. Although MabB4 was raised to PKX, it appears to stain some of the sporogonic stages of Sphaerospora spp. but not C. shasta. The results of the lectin and monoclonal staining of PKX and Sphaerospora oncorhynchi indicated that further work was required to prove or disprove the hypothesis that PKX and S, oncorhynchi are the same organism. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Spora ENVELOPE(17.220,17.220,69.504,69.504) Journal of Fish Diseases 19 1 55 63 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The results of comparative immunohisto‐chemical staining studies with two lectin probes, GS‐I and SBA, and five monoclonal antibodies raised against the PKX myxosporean are presented. In order to investigate the postulated link between PKX and Sphaerospora spp., the lectins and monoclonal antibodies were tested against PKX, Sphaerospora renicola, Sphaerospora oncorhynchi, Sphaerospora elegam, Sphaerospora truttae and Sphaero‐spora sp. from Atlantic salmon, Salmo salar L., and Ceratomyxa shasta. SBA appears a useful tool for tbe diagnosis of all Sphaerospora spp. infections and was also positive for C. shasta . GS‐I and monoclonal antibodies (12, C5, D4 and A3) appear to be specific for both the extrasporogonic and sporogonic stages of PKX. Although MabB4 was raised to PKX, it appears to stain some of the sporogonic stages of Sphaerospora spp. but not C. shasta. The results of the lectin and monoclonal staining of PKX and Sphaerospora oncorhynchi indicated that further work was required to prove or disprove the hypothesis that PKX and S, oncorhynchi are the same organism. |
format |
Article in Journal/Newspaper |
author |
de Mateo, M Marin McGeorge, J Morris, D Kent, M L |
spellingShingle |
de Mateo, M Marin McGeorge, J Morris, D Kent, M L Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
author_facet |
de Mateo, M Marin McGeorge, J Morris, D Kent, M L |
author_sort |
de Mateo, M Marin |
title |
Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
title_short |
Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
title_full |
Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
title_fullStr |
Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
title_full_unstemmed |
Comparative studies of PKX and Sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
title_sort |
comparative studies of pkx and sphaerospora spp. from salmonids using lectin and monoclonal antibody staining techniques |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1996.tb00120.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1996.tb00120.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1996.tb00120.x |
long_lat |
ENVELOPE(17.220,17.220,69.504,69.504) |
geographic |
Spora |
geographic_facet |
Spora |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 19, issue 1, page 55-63 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1996.tb00120.x |
container_title |
Journal of Fish Diseases |
container_volume |
19 |
container_issue |
1 |
container_start_page |
55 |
op_container_end_page |
63 |
_version_ |
1800748260801904640 |