The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro
Abstract. The stimulatory effect of LPS ( Vibrio anguillarum ), laminaran and sulphated laminaran, aqueous soluble β(1,6)‐branched β(l,3)‐D‐glucan obtained from Laminaria hyperborea , on head kidney macrophages of Atlantic salmon, Salmo salar L., is reported. The macrophages, after stimulation with...
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crwiley:10.1111/j.1365-2761.1995.tb00275.x 2024-06-23T07:51:15+00:00 The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro DALMO, R. A. SELJELID, R. 1995 http://dx.doi.org/10.1111/j.1365-2761.1995.tb00275.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1995.tb00275.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1995.tb00275.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 18, issue 2, page 175-185 ISSN 0140-7775 1365-2761 journal-article 1995 crwiley https://doi.org/10.1111/j.1365-2761.1995.tb00275.x 2024-06-06T04:22:09Z Abstract. The stimulatory effect of LPS ( Vibrio anguillarum ), laminaran and sulphated laminaran, aqueous soluble β(1,6)‐branched β(l,3)‐D‐glucan obtained from Laminaria hyperborea , on head kidney macrophages of Atlantic salmon, Salmo salar L., is reported. The macrophages, after stimulation with LPS or laminaran, showed pronounced spreading, membrane ruffling and increased organellc content when examined by light microscopy. LPS stimulation induced enhanced phagocytic and pinocytic activity, higher intraccllular production of superoxide anion, and higher activity of acid phosphatase compared to control cells. Native laminaran stimulated the cells to pinocytose more fluid phase, increase the intracellular production of superoxide anion and to elevate the activity of acid phosphatase. Sulphated laminaran induced higher production of superoxide anion and higher activity of acid phosphatase by macrophages than in control cells. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 18 2 175 185 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract. The stimulatory effect of LPS ( Vibrio anguillarum ), laminaran and sulphated laminaran, aqueous soluble β(1,6)‐branched β(l,3)‐D‐glucan obtained from Laminaria hyperborea , on head kidney macrophages of Atlantic salmon, Salmo salar L., is reported. The macrophages, after stimulation with LPS or laminaran, showed pronounced spreading, membrane ruffling and increased organellc content when examined by light microscopy. LPS stimulation induced enhanced phagocytic and pinocytic activity, higher intraccllular production of superoxide anion, and higher activity of acid phosphatase compared to control cells. Native laminaran stimulated the cells to pinocytose more fluid phase, increase the intracellular production of superoxide anion and to elevate the activity of acid phosphatase. Sulphated laminaran induced higher production of superoxide anion and higher activity of acid phosphatase by macrophages than in control cells. |
format |
Article in Journal/Newspaper |
author |
DALMO, R. A. SELJELID, R. |
spellingShingle |
DALMO, R. A. SELJELID, R. The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
author_facet |
DALMO, R. A. SELJELID, R. |
author_sort |
DALMO, R. A. |
title |
The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
title_short |
The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
title_full |
The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
title_fullStr |
The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
title_full_unstemmed |
The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(l,3)‐D‐glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro |
title_sort |
immunomodulatory effect of lps, laminaran and sulphated laminaran [β(l,3)‐d‐glucan] on atlantic salmon, salmo salar l., macrophages in vitro |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1995.tb00275.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1995.tb00275.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1995.tb00275.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 18, issue 2, page 175-185 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1995.tb00275.x |
container_title |
Journal of Fish Diseases |
container_volume |
18 |
container_issue |
2 |
container_start_page |
175 |
op_container_end_page |
185 |
_version_ |
1802642291230244864 |