Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L.
Abstract. Blood and head kidney (HK) leucocytes were isolated from Atlantic salmon, Salmo salar L., carrying infectious pancreatic necrosis virus (IPNV), and the cells were separated into adherent and non‐adherent populations. Significant increases in both intra‐ and extracellular IPNV titres, and i...
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crwiley:10.1111/j.1365-2761.1995.tb00272.x 2024-06-02T08:03:24+00:00 Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. JOHANSEN, L.‐H. SOMMER, A.‐I. 1995 http://dx.doi.org/10.1111/j.1365-2761.1995.tb00272.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1995.tb00272.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1995.tb00272.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 18, issue 2, page 147-156 ISSN 0140-7775 1365-2761 journal-article 1995 crwiley https://doi.org/10.1111/j.1365-2761.1995.tb00272.x 2024-05-03T10:51:28Z Abstract. Blood and head kidney (HK) leucocytes were isolated from Atlantic salmon, Salmo salar L., carrying infectious pancreatic necrosis virus (IPNV), and the cells were separated into adherent and non‐adherent populations. Significant increases in both intra‐ and extracellular IPNV titres, and in the number of IPNV‐positive fluorescent cells were detected in adherent HK leucocytes during 7 days in culture, and demonstrated that IPNV multiplied in these cells. Infectious virus was not detected in culture medium collected from blood leucoeytes, and only occasionally, in very low titres, from non‐adherent HK leucocytes. No IPNV‐positive fluorescent cells were detected in these cell populations. IPNV infection of adherent leucocytes isolated from non‐carrier fish indicated that adherent blood leucocytes (mainly monocytes) could become productively infected in vitro , but to a lesser degree than adherent HK leucocytes (mainly macrophages). The present results suggest a major role for adherent HK leucocytes in maintaining the IPNV carder state in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 18 2 147 156 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract. Blood and head kidney (HK) leucocytes were isolated from Atlantic salmon, Salmo salar L., carrying infectious pancreatic necrosis virus (IPNV), and the cells were separated into adherent and non‐adherent populations. Significant increases in both intra‐ and extracellular IPNV titres, and in the number of IPNV‐positive fluorescent cells were detected in adherent HK leucocytes during 7 days in culture, and demonstrated that IPNV multiplied in these cells. Infectious virus was not detected in culture medium collected from blood leucoeytes, and only occasionally, in very low titres, from non‐adherent HK leucocytes. No IPNV‐positive fluorescent cells were detected in these cell populations. IPNV infection of adherent leucocytes isolated from non‐carrier fish indicated that adherent blood leucocytes (mainly monocytes) could become productively infected in vitro , but to a lesser degree than adherent HK leucocytes (mainly macrophages). The present results suggest a major role for adherent HK leucocytes in maintaining the IPNV carder state in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
JOHANSEN, L.‐H. SOMMER, A.‐I. |
spellingShingle |
JOHANSEN, L.‐H. SOMMER, A.‐I. Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
author_facet |
JOHANSEN, L.‐H. SOMMER, A.‐I. |
author_sort |
JOHANSEN, L.‐H. |
title |
Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
title_short |
Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
title_full |
Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
title_fullStr |
Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. |
title_sort |
multiplication of infectious pancreatic necrosis virus (ipnv) in head kidney and blood leucocytes isolated from atlantic salmon, salmo salar l. |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1995.tb00272.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1995.tb00272.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1995.tb00272.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 18, issue 2, page 147-156 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1995.tb00272.x |
container_title |
Journal of Fish Diseases |
container_volume |
18 |
container_issue |
2 |
container_start_page |
147 |
op_container_end_page |
156 |
_version_ |
1800747916226199552 |