The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L.
Abstract. Western blot (WB) and electroimmunotransfer blot (EITB) methods were used to monitor bacterial soluble antigens and scroconversion profiles, respectively, in Atlantic salmon, Salmo salar L., experimentally challenged with Renibacterium salmoninarum. The two challenged populations exhibited...
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1992
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Online Access: | http://dx.doi.org/10.1111/j.1365-2761.1992.tb00659.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1992.tb00659.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1992.tb00659.x |
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crwiley:10.1111/j.1365-2761.1992.tb00659.x 2023-12-03T10:19:21+01:00 The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. OLIVIER, G. GRIFFITHS, S. G. FILDES, J. LYNCH, W. H. 1992 http://dx.doi.org/10.1111/j.1365-2761.1992.tb00659.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1992.tb00659.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1992.tb00659.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 15, issue 3, page 229-241 ISSN 0140-7775 1365-2761 Veterinary (miscellaneous) Aquatic Science journal-article 1992 crwiley https://doi.org/10.1111/j.1365-2761.1992.tb00659.x 2023-11-09T13:22:21Z Abstract. Western blot (WB) and electroimmunotransfer blot (EITB) methods were used to monitor bacterial soluble antigens and scroconversion profiles, respectively, in Atlantic salmon, Salmo salar L., experimentally challenged with Renibacterium salmoninarum. The two challenged populations exhibited differences in susceptibility to R. salmoninarum permitting an evaluation of the methods in fish populations harbouring different levels of the pathogen, WB detection of R. salmoninarum soluble antigens in serum provided a sensitive method for detecting pathogen presence during the active infection stages. For comparison, results for drop‐plate culture and direct fluorescent antibody techniques were obtained. During the recovery phase, surviving fish were negative for the pathogen by these methods. However, >90% of challenged fish sampled from early infection stages to the end of the experimental period (141 days post‐infection) were scropositive by the EITB assay for Atlantic salmon immunoglobulin reactive with R. salmoninarum soluble antigens. The results suggest that these methods for detecting R. salmoninarum or its soluble antigens may be useful during active infections. However, asymptomatic fish populations exposed to the pathogen, perhaps having recovered from an active infection and representing potential disease carrier sources, may be more readily identified by antigen‐specific antibodies using methods such as the EITB assay. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library (via Crossref) Journal of Fish Diseases 15 3 229 241 |
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Open Polar |
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Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Veterinary (miscellaneous) Aquatic Science |
spellingShingle |
Veterinary (miscellaneous) Aquatic Science OLIVIER, G. GRIFFITHS, S. G. FILDES, J. LYNCH, W. H. The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
topic_facet |
Veterinary (miscellaneous) Aquatic Science |
description |
Abstract. Western blot (WB) and electroimmunotransfer blot (EITB) methods were used to monitor bacterial soluble antigens and scroconversion profiles, respectively, in Atlantic salmon, Salmo salar L., experimentally challenged with Renibacterium salmoninarum. The two challenged populations exhibited differences in susceptibility to R. salmoninarum permitting an evaluation of the methods in fish populations harbouring different levels of the pathogen, WB detection of R. salmoninarum soluble antigens in serum provided a sensitive method for detecting pathogen presence during the active infection stages. For comparison, results for drop‐plate culture and direct fluorescent antibody techniques were obtained. During the recovery phase, surviving fish were negative for the pathogen by these methods. However, >90% of challenged fish sampled from early infection stages to the end of the experimental period (141 days post‐infection) were scropositive by the EITB assay for Atlantic salmon immunoglobulin reactive with R. salmoninarum soluble antigens. The results suggest that these methods for detecting R. salmoninarum or its soluble antigens may be useful during active infections. However, asymptomatic fish populations exposed to the pathogen, perhaps having recovered from an active infection and representing potential disease carrier sources, may be more readily identified by antigen‐specific antibodies using methods such as the EITB assay. |
format |
Article in Journal/Newspaper |
author |
OLIVIER, G. GRIFFITHS, S. G. FILDES, J. LYNCH, W. H. |
author_facet |
OLIVIER, G. GRIFFITHS, S. G. FILDES, J. LYNCH, W. H. |
author_sort |
OLIVIER, G. |
title |
The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
title_short |
The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
title_full |
The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
title_fullStr |
The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
The use of Western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. |
title_sort |
use of western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged atlantic salmon, salmo salar l. |
publisher |
Wiley |
publishDate |
1992 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1992.tb00659.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1992.tb00659.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1992.tb00659.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 15, issue 3, page 229-241 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1992.tb00659.x |
container_title |
Journal of Fish Diseases |
container_volume |
15 |
container_issue |
3 |
container_start_page |
229 |
op_container_end_page |
241 |
_version_ |
1784266577386930176 |