Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish

Abstract. The effectiveness of three different growth media KDM‐2, S‐KDM and S‐KDM‐C for primary isolation of Renibacterium salmoninarum was examined over a 14‐week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at...

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Published in:Journal of Fish Diseases
Main Authors: GUDMUNDSDóTTIR, S., HELGASON, S., BENEDIKTSDOTTIR, E.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1991
Subjects:
Online Access:http://dx.doi.org/10.1111/j.1365-2761.1991.tb00579.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1991.tb00579.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1991.tb00579.x
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spelling crwiley:10.1111/j.1365-2761.1991.tb00579.x 2024-06-02T08:03:29+00:00 Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish GUDMUNDSDóTTIR, S. HELGASON, S. BENEDIKTSDOTTIR, E. 1991 http://dx.doi.org/10.1111/j.1365-2761.1991.tb00579.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1991.tb00579.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1991.tb00579.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 14, issue 1, page 89-96 ISSN 0140-7775 1365-2761 journal-article 1991 crwiley https://doi.org/10.1111/j.1365-2761.1991.tb00579.x 2024-05-03T11:40:18Z Abstract. The effectiveness of three different growth media KDM‐2, S‐KDM and S‐KDM‐C for primary isolation of Renibacterium salmoninarum was examined over a 14‐week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S‐KDM supplemented with lamb serum and S‐KDM‐C supplemented with activated charcoal. The third medium was the non‐selective serum supplemented KDM‐2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S‐KDM, 60% on S‐KDM‐C and 35% on KDM‐2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 14 1 89 96
institution Open Polar
collection Wiley Online Library
op_collection_id crwiley
language English
description Abstract. The effectiveness of three different growth media KDM‐2, S‐KDM and S‐KDM‐C for primary isolation of Renibacterium salmoninarum was examined over a 14‐week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S‐KDM supplemented with lamb serum and S‐KDM‐C supplemented with activated charcoal. The third medium was the non‐selective serum supplemented KDM‐2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S‐KDM, 60% on S‐KDM‐C and 35% on KDM‐2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal.
format Article in Journal/Newspaper
author GUDMUNDSDóTTIR, S.
HELGASON, S.
BENEDIKTSDOTTIR, E.
spellingShingle GUDMUNDSDóTTIR, S.
HELGASON, S.
BENEDIKTSDOTTIR, E.
Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
author_facet GUDMUNDSDóTTIR, S.
HELGASON, S.
BENEDIKTSDOTTIR, E.
author_sort GUDMUNDSDóTTIR, S.
title Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
title_short Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
title_full Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
title_fullStr Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
title_full_unstemmed Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
title_sort comparison of the effectiveness of three different growth media for primary isolation of renibacterium salmoninarum from atlantic salmon, salmo salar l., broodfish
publisher Wiley
publishDate 1991
url http://dx.doi.org/10.1111/j.1365-2761.1991.tb00579.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1991.tb00579.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1991.tb00579.x
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Journal of Fish Diseases
volume 14, issue 1, page 89-96
ISSN 0140-7775 1365-2761
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1111/j.1365-2761.1991.tb00579.x
container_title Journal of Fish Diseases
container_volume 14
container_issue 1
container_start_page 89
op_container_end_page 96
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