Isolation and characterization of a surface layer antigen from Vibrio salmonicida
Abstract. A cell surface product (VS‐P1) of Vibrio salmonicida has been purified from culture supernatant by a combination of extensive dialysis, filtration and centrifugation, as well as by salt precipitation and hydrophobic chromatography. SDS‐PAGE analysis showed that the monomeric form of the an...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1988
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1365-2761.1988.tb00540.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1988.tb00540.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1988.tb00540.x |
id |
crwiley:10.1111/j.1365-2761.1988.tb00540.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1365-2761.1988.tb00540.x 2024-06-23T07:51:21+00:00 Isolation and characterization of a surface layer antigen from Vibrio salmonicida HJELMELAND, K. STENSVÅG, K. JØRGENSEN, T. ESPELID, S. 1988 http://dx.doi.org/10.1111/j.1365-2761.1988.tb00540.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1988.tb00540.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1988.tb00540.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 11, issue 3, page 197-205 ISSN 0140-7775 1365-2761 journal-article 1988 crwiley https://doi.org/10.1111/j.1365-2761.1988.tb00540.x 2024-06-06T04:23:42Z Abstract. A cell surface product (VS‐P1) of Vibrio salmonicida has been purified from culture supernatant by a combination of extensive dialysis, filtration and centrifugation, as well as by salt precipitation and hydrophobic chromatography. SDS‐PAGE analysis showed that the monomeric form of the antigen is a single polypeptide with an apparent molecular weight of 40000. Size exclusion HPLC of purified VS‐P1 as well as VS‐Pl‐containing fish serum revealed, however, oligomeric forms in the range from 300000 to more than 700000 daltons. The antigen contained 6% carbohydrate and several isolectric forms were distinguishable when analysed on an analytical isoelectric focusing electrophoresis system. A ‘sandwich’ ELISA, utilizing polyclonal antibodies, was developed for screening sera from both healthy and moribund Atlantic salmon for the presence of the VS‐P1 antigen. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Fish Diseases 11 3 197 205 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract. A cell surface product (VS‐P1) of Vibrio salmonicida has been purified from culture supernatant by a combination of extensive dialysis, filtration and centrifugation, as well as by salt precipitation and hydrophobic chromatography. SDS‐PAGE analysis showed that the monomeric form of the antigen is a single polypeptide with an apparent molecular weight of 40000. Size exclusion HPLC of purified VS‐P1 as well as VS‐Pl‐containing fish serum revealed, however, oligomeric forms in the range from 300000 to more than 700000 daltons. The antigen contained 6% carbohydrate and several isolectric forms were distinguishable when analysed on an analytical isoelectric focusing electrophoresis system. A ‘sandwich’ ELISA, utilizing polyclonal antibodies, was developed for screening sera from both healthy and moribund Atlantic salmon for the presence of the VS‐P1 antigen. |
format |
Article in Journal/Newspaper |
author |
HJELMELAND, K. STENSVÅG, K. JØRGENSEN, T. ESPELID, S. |
spellingShingle |
HJELMELAND, K. STENSVÅG, K. JØRGENSEN, T. ESPELID, S. Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
author_facet |
HJELMELAND, K. STENSVÅG, K. JØRGENSEN, T. ESPELID, S. |
author_sort |
HJELMELAND, K. |
title |
Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
title_short |
Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
title_full |
Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
title_fullStr |
Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
title_full_unstemmed |
Isolation and characterization of a surface layer antigen from Vibrio salmonicida |
title_sort |
isolation and characterization of a surface layer antigen from vibrio salmonicida |
publisher |
Wiley |
publishDate |
1988 |
url |
http://dx.doi.org/10.1111/j.1365-2761.1988.tb00540.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.1988.tb00540.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.1988.tb00540.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases volume 11, issue 3, page 197-205 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2761.1988.tb00540.x |
container_title |
Journal of Fish Diseases |
container_volume |
11 |
container_issue |
3 |
container_start_page |
197 |
op_container_end_page |
205 |
_version_ |
1802642429863526400 |