Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting
ABSTRACT Cross‐linking of myofibrillar proteins extracted from cod ( Gadus morhua) , herring ( Clupea harengus ) and silver hake ( Merluccius bilinearis ) was studied in 0.6M NaCl, pH 6.5 at 40°C and evaluated turbidimetrically and by SDS polyacrylamide gel electrophoresis coupled with l‐ethyl‐3‐(3‐...
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Online Access: | http://dx.doi.org/10.1111/j.1365-2621.1992.tb14320.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1992.tb14320.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1992.tb14320.x/fullpdf |
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crwiley:10.1111/j.1365-2621.1992.tb14320.x 2024-06-23T07:52:56+00:00 Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting CHAN, J.K. GILL, T.A. PAULSON, AT. 1992 http://dx.doi.org/10.1111/j.1365-2621.1992.tb14320.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1992.tb14320.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1992.tb14320.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 57, issue 4, page 906-912 ISSN 0022-1147 1750-3841 journal-article 1992 crwiley https://doi.org/10.1111/j.1365-2621.1992.tb14320.x 2024-06-11T04:42:05Z ABSTRACT Cross‐linking of myofibrillar proteins extracted from cod ( Gadus morhua) , herring ( Clupea harengus ) and silver hake ( Merluccius bilinearis ) was studied in 0.6M NaCl, pH 6.5 at 40°C and evaluated turbidimetrically and by SDS polyacrylamide gel electrophoresis coupled with l‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide as a zero‐length crosslinker. Turbidities of heat‐treated cod and silver hake myofibril/myosin solutions were significantly higher than those of herring. Electrophoretic results showed that the myosin heavy chain (MHC) was the principal myofibrillar protein cross‐linked to form a polymerized complex during the heat treatment. Cross‐linking ability of MHC from the three fish species was different; herring MHC formed only small polymers (n≦3) but cod and silver hake MHC formed both small and large polymers (n≦6). Article in Journal/Newspaper Gadus morhua Wiley Online Library Hake ENVELOPE(15.612,15.612,66.797,66.797) Journal of Food Science 57 4 906 912 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
ABSTRACT Cross‐linking of myofibrillar proteins extracted from cod ( Gadus morhua) , herring ( Clupea harengus ) and silver hake ( Merluccius bilinearis ) was studied in 0.6M NaCl, pH 6.5 at 40°C and evaluated turbidimetrically and by SDS polyacrylamide gel electrophoresis coupled with l‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide as a zero‐length crosslinker. Turbidities of heat‐treated cod and silver hake myofibril/myosin solutions were significantly higher than those of herring. Electrophoretic results showed that the myosin heavy chain (MHC) was the principal myofibrillar protein cross‐linked to form a polymerized complex during the heat treatment. Cross‐linking ability of MHC from the three fish species was different; herring MHC formed only small polymers (n≦3) but cod and silver hake MHC formed both small and large polymers (n≦6). |
format |
Article in Journal/Newspaper |
author |
CHAN, J.K. GILL, T.A. PAULSON, AT. |
spellingShingle |
CHAN, J.K. GILL, T.A. PAULSON, AT. Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
author_facet |
CHAN, J.K. GILL, T.A. PAULSON, AT. |
author_sort |
CHAN, J.K. |
title |
Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
title_short |
Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
title_full |
Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
title_fullStr |
Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
title_full_unstemmed |
Cross‐Linking of Myosin Heavy Chains from Cod, Herring and Silver Hake During Thermal Setting |
title_sort |
cross‐linking of myosin heavy chains from cod, herring and silver hake during thermal setting |
publisher |
Wiley |
publishDate |
1992 |
url |
http://dx.doi.org/10.1111/j.1365-2621.1992.tb14320.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1992.tb14320.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1992.tb14320.x/fullpdf |
long_lat |
ENVELOPE(15.612,15.612,66.797,66.797) |
geographic |
Hake |
geographic_facet |
Hake |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Journal of Food Science volume 57, issue 4, page 906-912 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2621.1992.tb14320.x |
container_title |
Journal of Food Science |
container_volume |
57 |
container_issue |
4 |
container_start_page |
906 |
op_container_end_page |
912 |
_version_ |
1802644381827596288 |