Electrophoretic Identification of Fish Species Used in Surimi Products

ABSTRACT Both sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and urea gel isoelectric focusing (IEF) were used to identify species‐specific protein bands of raw and cooked fish and surimi samples from Alaska pollock ( Theragra chalcogramma ) and red hake ( Urophycis chuss ). In...

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Bibliographic Details
Published in:Journal of Food Science
Main Authors: AN, H., WEI, C. I., ZHAO, J., MARSHALL, M.R., LEE, C.M.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1989
Subjects:
Hake
alaska pollock
Theragra chalcogramma
Alaska
Online Access:http://dx.doi.org/10.1111/j.1365-2621.1989.tb03055.x
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Summary:ABSTRACT Both sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and urea gel isoelectric focusing (IEF) were used to identify species‐specific protein bands of raw and cooked fish and surimi samples from Alaska pollock ( Theragra chalcogramma ) and red hake ( Urophycis chuss ). In raw samples, species‐specific bands were found in the water extracts, while in cooked samples 1% SDS and 8M urea extracts were more effective for species identification in both fish and surimi.

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