Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua)
ABSTRACT Incubation of microsomes prepared from myotomal tissue of Atlantic cod yielded detectable phospholipase activity following incubation with 1,2‐dipalmitoyl‐9,10‐[ 3 H]‐phosphatidylcholine over a period of 24 hr. No significant incidence of nonenzymatic hydrolysis was found to accompany the d...
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1987
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Online Access: | http://dx.doi.org/10.1111/j.1365-2621.1987.tb14042.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1987.tb14042.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1987.tb14042.x/fullpdf |
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crwiley:10.1111/j.1365-2621.1987.tb14042.x 2024-06-02T08:03:07+00:00 Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) CHAWLA, PARVEEN ABLETT, RICHARD F. 1987 http://dx.doi.org/10.1111/j.1365-2621.1987.tb14042.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1987.tb14042.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1987.tb14042.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 52, issue 5, page 1194-1197 ISSN 0022-1147 1750-3841 journal-article 1987 crwiley https://doi.org/10.1111/j.1365-2621.1987.tb14042.x 2024-05-03T11:29:18Z ABSTRACT Incubation of microsomes prepared from myotomal tissue of Atlantic cod yielded detectable phospholipase activity following incubation with 1,2‐dipalmitoyl‐9,10‐[ 3 H]‐phosphatidylcholine over a period of 24 hr. No significant incidence of nonenzymatic hydrolysis was found to accompany the determined phospholipase activity. Phospholipid hydrolysis increased linearly in the presence of increasing levels of microsomal protein (0–4 mg). Optimal phospholipase enzyme activity was determined following incubation at 20°C and maximal activity was determined in the presence of 0.1 nmole unlabelled lecithin substrate. The myotomal microsome preparation of Atlantic cod proved to be a suitable model for direct evaluation of phospholipase and perhaps might be useful in evaluation of frozen storage impact on the resultant integrity of the enzyme system. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Journal of Food Science 52 5 1194 1197 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
ABSTRACT Incubation of microsomes prepared from myotomal tissue of Atlantic cod yielded detectable phospholipase activity following incubation with 1,2‐dipalmitoyl‐9,10‐[ 3 H]‐phosphatidylcholine over a period of 24 hr. No significant incidence of nonenzymatic hydrolysis was found to accompany the determined phospholipase activity. Phospholipid hydrolysis increased linearly in the presence of increasing levels of microsomal protein (0–4 mg). Optimal phospholipase enzyme activity was determined following incubation at 20°C and maximal activity was determined in the presence of 0.1 nmole unlabelled lecithin substrate. The myotomal microsome preparation of Atlantic cod proved to be a suitable model for direct evaluation of phospholipase and perhaps might be useful in evaluation of frozen storage impact on the resultant integrity of the enzyme system. |
format |
Article in Journal/Newspaper |
author |
CHAWLA, PARVEEN ABLETT, RICHARD F. |
spellingShingle |
CHAWLA, PARVEEN ABLETT, RICHARD F. Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
author_facet |
CHAWLA, PARVEEN ABLETT, RICHARD F. |
author_sort |
CHAWLA, PARVEEN |
title |
Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
title_short |
Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
title_full |
Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
title_fullStr |
Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
title_full_unstemmed |
Detection of Microsomal Phospholipase Activity in Myotomal Tissue of Atlantic Cod ( Gadus morhua) |
title_sort |
detection of microsomal phospholipase activity in myotomal tissue of atlantic cod ( gadus morhua) |
publisher |
Wiley |
publishDate |
1987 |
url |
http://dx.doi.org/10.1111/j.1365-2621.1987.tb14042.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1987.tb14042.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1987.tb14042.x/fullpdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of Food Science volume 52, issue 5, page 1194-1197 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2621.1987.tb14042.x |
container_title |
Journal of Food Science |
container_volume |
52 |
container_issue |
5 |
container_start_page |
1194 |
op_container_end_page |
1197 |
_version_ |
1800747583829704704 |