Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties
ABSTRACT The presence of neutral and alkaline proteinases in the extract from Antarctic krill, Euphausia superba was confirmed. In addition to these enzymes, acid proteinases were found in the extract from E. superba and the two major acid proteinases, A and B, were purified 80–140 fold. The molecul...
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crwiley:10.1111/j.1365-2621.1981.tb04510.x 2024-06-02T07:55:31+00:00 Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties KIMOTO, K. THANH, V. V. MURAKAMI, K. 1981 http://dx.doi.org/10.1111/j.1365-2621.1981.tb04510.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1981.tb04510.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1981.tb04510.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 46, issue 6, page 1881-1884 ISSN 0022-1147 1750-3841 journal-article 1981 crwiley https://doi.org/10.1111/j.1365-2621.1981.tb04510.x 2024-05-06T07:01:26Z ABSTRACT The presence of neutral and alkaline proteinases in the extract from Antarctic krill, Euphausia superba was confirmed. In addition to these enzymes, acid proteinases were found in the extract from E. superba and the two major acid proteinases, A and B, were purified 80–140 fold. The molecular weights of acid proteinases A and B were estimated by gel filtration on Sephadex G‐100 to be approximately 45,000 and 64,000, respectively. The optimal pH of enzymes A and B was 3.0 toward hemoglobin. They were partially inhibited by acid proteinase specific reagents: diazoacethyl‐DL‐norleucine methylester (DAN) and 1,2epoxy‐3‐( p ‐nitrophenoxy) propane (EPNP). However, enzyme B was less sensitive to pepstatin while enzyme A was inhibited by pepstatin like almost all other acid proteinases. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Wiley Online Library Antarctic Journal of Food Science 46 6 1881 1884 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
ABSTRACT The presence of neutral and alkaline proteinases in the extract from Antarctic krill, Euphausia superba was confirmed. In addition to these enzymes, acid proteinases were found in the extract from E. superba and the two major acid proteinases, A and B, were purified 80–140 fold. The molecular weights of acid proteinases A and B were estimated by gel filtration on Sephadex G‐100 to be approximately 45,000 and 64,000, respectively. The optimal pH of enzymes A and B was 3.0 toward hemoglobin. They were partially inhibited by acid proteinase specific reagents: diazoacethyl‐DL‐norleucine methylester (DAN) and 1,2epoxy‐3‐( p ‐nitrophenoxy) propane (EPNP). However, enzyme B was less sensitive to pepstatin while enzyme A was inhibited by pepstatin like almost all other acid proteinases. |
format |
Article in Journal/Newspaper |
author |
KIMOTO, K. THANH, V. V. MURAKAMI, K. |
spellingShingle |
KIMOTO, K. THANH, V. V. MURAKAMI, K. Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
author_facet |
KIMOTO, K. THANH, V. V. MURAKAMI, K. |
author_sort |
KIMOTO, K. |
title |
Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
title_short |
Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
title_full |
Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
title_fullStr |
Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
title_full_unstemmed |
Acid Proteinases from Antarctic Krill, Euphausia superb: Partial Purification and Some Properties |
title_sort |
acid proteinases from antarctic krill, euphausia superb: partial purification and some properties |
publisher |
Wiley |
publishDate |
1981 |
url |
http://dx.doi.org/10.1111/j.1365-2621.1981.tb04510.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1981.tb04510.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1981.tb04510.x/fullpdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
Journal of Food Science volume 46, issue 6, page 1881-1884 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2621.1981.tb04510.x |
container_title |
Journal of Food Science |
container_volume |
46 |
container_issue |
6 |
container_start_page |
1881 |
op_container_end_page |
1884 |
_version_ |
1800749315880124416 |