Rapid detection of fungal endophytes in grasses for large‐scale studies
Summary Standard visual screening methods for determining the qualitative and quantitative presence of fungal endophytes are too time‐consuming for large‐scale ecological studies. We investigated whether commercially available immunoblot kits, using monoclonal antibody techniques and designed for ra...
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Online Access: | http://dx.doi.org/10.1111/j.1365-2435.2006.01150.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2435.2006.01150.x https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2435.2006.01150.x |
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crwiley:10.1111/j.1365-2435.2006.01150.x 2024-06-02T08:02:23+00:00 Rapid detection of fungal endophytes in grasses for large‐scale studies KOH, S. VICARI, M. BALL, J. P. RAKOCEVIC, T. ZAHEER, S. HIK, D. S. BAZELY, D. R. 2006 http://dx.doi.org/10.1111/j.1365-2435.2006.01150.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2435.2006.01150.x https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2435.2006.01150.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Functional Ecology volume 20, issue 4, page 736-742 ISSN 0269-8463 1365-2435 journal-article 2006 crwiley https://doi.org/10.1111/j.1365-2435.2006.01150.x 2024-05-03T11:48:01Z Summary Standard visual screening methods for determining the qualitative and quantitative presence of fungal endophytes are too time‐consuming for large‐scale ecological studies. We investigated whether commercially available immunoblot kits, using monoclonal antibody techniques and designed for rapid‐screening of the presence of Neotyphodium endophytes in fresh samples of the pasture grasses Festuca arundinacea and Lolium perenne , could be used for Neotyphodium detection using other grasses and preserved samples. We also determined whether immunoblot kits could provide quantitative information about the amount of Neotyphodium in the grass. The kits accurately detected endophyte presence in F. rubra , F. ovina , F. pratensis and F. altaica , in both preserved samples (dried and fixed), including 12‐year‐old stored, dried samples of F. rubra . Endophytes were detected in 7‐day‐old seedlings of Lolium perenne , 3 days (30%) earlier than previously recognized. The intensity of the coloured tissue prints on scanned immunoblot cards was significantly positively correlated with hyphal density, demonstrating a previously unrecognized accurate quantitative application. These findings greatly reduce logistical barriers to large‐scale field research into the broader ecological significance of Neotyphodium in temperate and arctic grasses in non‐agricultural ecosystems (particularly in remote areas) and suggest potential for estimating historical infection rates using stored and herbarium specimens. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Functional Ecology 20 4 736 742 |
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Wiley Online Library |
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English |
description |
Summary Standard visual screening methods for determining the qualitative and quantitative presence of fungal endophytes are too time‐consuming for large‐scale ecological studies. We investigated whether commercially available immunoblot kits, using monoclonal antibody techniques and designed for rapid‐screening of the presence of Neotyphodium endophytes in fresh samples of the pasture grasses Festuca arundinacea and Lolium perenne , could be used for Neotyphodium detection using other grasses and preserved samples. We also determined whether immunoblot kits could provide quantitative information about the amount of Neotyphodium in the grass. The kits accurately detected endophyte presence in F. rubra , F. ovina , F. pratensis and F. altaica , in both preserved samples (dried and fixed), including 12‐year‐old stored, dried samples of F. rubra . Endophytes were detected in 7‐day‐old seedlings of Lolium perenne , 3 days (30%) earlier than previously recognized. The intensity of the coloured tissue prints on scanned immunoblot cards was significantly positively correlated with hyphal density, demonstrating a previously unrecognized accurate quantitative application. These findings greatly reduce logistical barriers to large‐scale field research into the broader ecological significance of Neotyphodium in temperate and arctic grasses in non‐agricultural ecosystems (particularly in remote areas) and suggest potential for estimating historical infection rates using stored and herbarium specimens. |
format |
Article in Journal/Newspaper |
author |
KOH, S. VICARI, M. BALL, J. P. RAKOCEVIC, T. ZAHEER, S. HIK, D. S. BAZELY, D. R. |
spellingShingle |
KOH, S. VICARI, M. BALL, J. P. RAKOCEVIC, T. ZAHEER, S. HIK, D. S. BAZELY, D. R. Rapid detection of fungal endophytes in grasses for large‐scale studies |
author_facet |
KOH, S. VICARI, M. BALL, J. P. RAKOCEVIC, T. ZAHEER, S. HIK, D. S. BAZELY, D. R. |
author_sort |
KOH, S. |
title |
Rapid detection of fungal endophytes in grasses for large‐scale studies |
title_short |
Rapid detection of fungal endophytes in grasses for large‐scale studies |
title_full |
Rapid detection of fungal endophytes in grasses for large‐scale studies |
title_fullStr |
Rapid detection of fungal endophytes in grasses for large‐scale studies |
title_full_unstemmed |
Rapid detection of fungal endophytes in grasses for large‐scale studies |
title_sort |
rapid detection of fungal endophytes in grasses for large‐scale studies |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1365-2435.2006.01150.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2435.2006.01150.x https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2435.2006.01150.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Functional Ecology volume 20, issue 4, page 736-742 ISSN 0269-8463 1365-2435 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2435.2006.01150.x |
container_title |
Functional Ecology |
container_volume |
20 |
container_issue |
4 |
container_start_page |
736 |
op_container_end_page |
742 |
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1800746877290807296 |