Microsatellite markers in common carp ( Cyprinus carpio L.)
Microsatellite markers of the poly (CA) type in common carp ( Cyprinus carpio L.) are described. Clones containing a (CA) repeat were isolated from a common carp genomic library and sequenced. The number of repeats found was high compared to mammals but comparable with other teleost fishes. Classifi...
Published in: | Animal Genetics |
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crwiley:10.1111/j.1365-2052.1997.00097.x 2024-06-23T07:51:05+00:00 Microsatellite markers in common carp ( Cyprinus carpio L.) Crooijmans, R P M A Poel, J J Van der Groenen, M A M Bierbooms, V A F Komen, J 1997 http://dx.doi.org/10.1111/j.1365-2052.1997.00097.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2052.1997.00097.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2052.1997.00097.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Animal Genetics volume 28, issue 2, page 129-134 ISSN 0268-9146 1365-2052 journal-article 1997 crwiley https://doi.org/10.1111/j.1365-2052.1997.00097.x 2024-06-04T06:48:51Z Microsatellite markers of the poly (CA) type in common carp ( Cyprinus carpio L.) are described. Clones containing a (CA) repeat were isolated from a common carp genomic library and sequenced. The number of repeats found was high compared to mammals but comparable with other teleost fishes. Classification of the repeats (perfect, imperfect and compound) are compared with the Atlantic cod ( Gadus morhua L.), rainbow trout ( Oncorhynchus mykiss ), and Atlantic salmon ( Salmo salar L.). A total of 41 primer sets were designed and tested for polymorphism on a test panel of eight animals (derived from outbred lines, inbred lines and gynogenetic clones). Thirty‐two markers were found to be polymorphic. The heterozygosity in the outbred animals was 60·4%, 51·1% in the inbred animals and 0% in the gynogenetic clones. The average number of alleles among the eight animals was 4·7 per marker. Six markers (18·8%) gave an additional polymorphic amplification product besides the polymorphic amplification product in the expected size range. The possibility that these loci are tetraploid is discussed. The polymorphic loci described for common carp will be valuable as genetic markers for use in population, breeding, and evolutionary studies. Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Salmo salar Wiley Online Library Animal Genetics 28 2 129 134 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Microsatellite markers of the poly (CA) type in common carp ( Cyprinus carpio L.) are described. Clones containing a (CA) repeat were isolated from a common carp genomic library and sequenced. The number of repeats found was high compared to mammals but comparable with other teleost fishes. Classification of the repeats (perfect, imperfect and compound) are compared with the Atlantic cod ( Gadus morhua L.), rainbow trout ( Oncorhynchus mykiss ), and Atlantic salmon ( Salmo salar L.). A total of 41 primer sets were designed and tested for polymorphism on a test panel of eight animals (derived from outbred lines, inbred lines and gynogenetic clones). Thirty‐two markers were found to be polymorphic. The heterozygosity in the outbred animals was 60·4%, 51·1% in the inbred animals and 0% in the gynogenetic clones. The average number of alleles among the eight animals was 4·7 per marker. Six markers (18·8%) gave an additional polymorphic amplification product besides the polymorphic amplification product in the expected size range. The possibility that these loci are tetraploid is discussed. The polymorphic loci described for common carp will be valuable as genetic markers for use in population, breeding, and evolutionary studies. |
format |
Article in Journal/Newspaper |
author |
Crooijmans, R P M A Poel, J J Van der Groenen, M A M Bierbooms, V A F Komen, J |
spellingShingle |
Crooijmans, R P M A Poel, J J Van der Groenen, M A M Bierbooms, V A F Komen, J Microsatellite markers in common carp ( Cyprinus carpio L.) |
author_facet |
Crooijmans, R P M A Poel, J J Van der Groenen, M A M Bierbooms, V A F Komen, J |
author_sort |
Crooijmans, R P M A |
title |
Microsatellite markers in common carp ( Cyprinus carpio L.) |
title_short |
Microsatellite markers in common carp ( Cyprinus carpio L.) |
title_full |
Microsatellite markers in common carp ( Cyprinus carpio L.) |
title_fullStr |
Microsatellite markers in common carp ( Cyprinus carpio L.) |
title_full_unstemmed |
Microsatellite markers in common carp ( Cyprinus carpio L.) |
title_sort |
microsatellite markers in common carp ( cyprinus carpio l.) |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1111/j.1365-2052.1997.00097.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2052.1997.00097.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2052.1997.00097.x |
genre |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
op_source |
Animal Genetics volume 28, issue 2, page 129-134 ISSN 0268-9146 1365-2052 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1365-2052.1997.00097.x |
container_title |
Animal Genetics |
container_volume |
28 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
134 |
_version_ |
1802642084575838208 |