Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species
Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average...
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crwiley:10.1111/j.1095-8649.2012.03442.x 2024-06-02T08:11:15+00:00 Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species Bernard, A. M. Feldheim, K. A. Shivji, M. S. 2012 http://dx.doi.org/10.1111/j.1095-8649.2012.03442.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2012.03442.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2012.03442.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 81, issue 5, page 1781-1786 ISSN 0022-1112 1095-8649 journal-article 2012 crwiley https://doi.org/10.1111/j.1095-8649.2012.03442.x 2024-05-03T11:34:28Z Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity ( H E ) of 0·09–0·94, and all loci conformed to Hardy–Weinberg expectations. Cross‐amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general. Article in Journal/Newspaper North Atlantic Wiley Online Library Journal of Fish Biology 81 5 1781 1786 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity ( H E ) of 0·09–0·94, and all loci conformed to Hardy–Weinberg expectations. Cross‐amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general. |
format |
Article in Journal/Newspaper |
author |
Bernard, A. M. Feldheim, K. A. Shivji, M. S. |
spellingShingle |
Bernard, A. M. Feldheim, K. A. Shivji, M. S. Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
author_facet |
Bernard, A. M. Feldheim, K. A. Shivji, M. S. |
author_sort |
Bernard, A. M. |
title |
Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
title_short |
Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
title_full |
Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
title_fullStr |
Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
title_full_unstemmed |
Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross‐species amplification among other istiophorid species |
title_sort |
development and characterization of 11 novel microsatellite loci for the roundscale spearfish tetrapturus georgii and their cross‐species amplification among other istiophorid species |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1111/j.1095-8649.2012.03442.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2012.03442.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2012.03442.x |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Journal of Fish Biology volume 81, issue 5, page 1781-1786 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.2012.03442.x |
container_title |
Journal of Fish Biology |
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81 |
container_issue |
5 |
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1781 |
op_container_end_page |
1786 |
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1800757332012957696 |