The effects of preservation on fish tissue stable isotope signatures
The effects of formalin and ethanol preservation on the δ 13 C and δ 15 N isotope signatures of Arctic charr Salvelinus alpinus muscle tissue were examined. The lipid content of the tissue samples studied ranged from 3·6 to 6·1% and was not correlated with the magnitude of observed isotopic shifts i...
Published in: | Journal of Fish Biology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2006
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1095-8649.2006.01226.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2006.01226.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2006.01226.x |
id |
crwiley:10.1111/j.1095-8649.2006.01226.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1095-8649.2006.01226.x 2024-06-23T07:48:55+00:00 The effects of preservation on fish tissue stable isotope signatures Kelly, B. Dempson, J. B. Power, M. 2006 http://dx.doi.org/10.1111/j.1095-8649.2006.01226.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2006.01226.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2006.01226.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 69, issue 6, page 1595-1611 ISSN 0022-1112 1095-8649 journal-article 2006 crwiley https://doi.org/10.1111/j.1095-8649.2006.01226.x 2024-06-13T04:24:08Z The effects of formalin and ethanol preservation on the δ 13 C and δ 15 N isotope signatures of Arctic charr Salvelinus alpinus muscle tissue were examined. The lipid content of the tissue samples studied ranged from 3·6 to 6·1% and was not correlated with the magnitude of observed isotopic shifts in preserved samples. Ethanol and formalin significantly depleted and enriched, respectively, the δ 13 C isotope signatures of preserved tissues when compared to control samples. Ethanol did not significantly enrich δ 15 N signatures in comparison to controls, whereas formalin did. A meta‐analysis of multiple species effects further demonstrated significant preservation effects in fish tissue. Statistical analysis of data obtained by correcting preserved tissue isotope signatures with literature, bootstrapped or meta‐analysis derived correction factors demonstrated significant differences between corrected and control sample isotope signatures or failure to produce a unity slope when the data sets were regressed against one another. Species‐specific, bootstrapped linear correction models resulted in no such errors. Results suggest that species‐specific correction methods should be used for fishes because of the known wide variation in fish tissue lipid content and composition. Accordingly, the use of pilot studies will be required to develop correction factors that properly adjust for preservation effects when interpreting temporal patterns in historic analyses of food webs. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Wiley Online Library Arctic Journal of Fish Biology 69 6 1595 1611 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The effects of formalin and ethanol preservation on the δ 13 C and δ 15 N isotope signatures of Arctic charr Salvelinus alpinus muscle tissue were examined. The lipid content of the tissue samples studied ranged from 3·6 to 6·1% and was not correlated with the magnitude of observed isotopic shifts in preserved samples. Ethanol and formalin significantly depleted and enriched, respectively, the δ 13 C isotope signatures of preserved tissues when compared to control samples. Ethanol did not significantly enrich δ 15 N signatures in comparison to controls, whereas formalin did. A meta‐analysis of multiple species effects further demonstrated significant preservation effects in fish tissue. Statistical analysis of data obtained by correcting preserved tissue isotope signatures with literature, bootstrapped or meta‐analysis derived correction factors demonstrated significant differences between corrected and control sample isotope signatures or failure to produce a unity slope when the data sets were regressed against one another. Species‐specific, bootstrapped linear correction models resulted in no such errors. Results suggest that species‐specific correction methods should be used for fishes because of the known wide variation in fish tissue lipid content and composition. Accordingly, the use of pilot studies will be required to develop correction factors that properly adjust for preservation effects when interpreting temporal patterns in historic analyses of food webs. |
format |
Article in Journal/Newspaper |
author |
Kelly, B. Dempson, J. B. Power, M. |
spellingShingle |
Kelly, B. Dempson, J. B. Power, M. The effects of preservation on fish tissue stable isotope signatures |
author_facet |
Kelly, B. Dempson, J. B. Power, M. |
author_sort |
Kelly, B. |
title |
The effects of preservation on fish tissue stable isotope signatures |
title_short |
The effects of preservation on fish tissue stable isotope signatures |
title_full |
The effects of preservation on fish tissue stable isotope signatures |
title_fullStr |
The effects of preservation on fish tissue stable isotope signatures |
title_full_unstemmed |
The effects of preservation on fish tissue stable isotope signatures |
title_sort |
effects of preservation on fish tissue stable isotope signatures |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1095-8649.2006.01226.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2006.01226.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2006.01226.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
Journal of Fish Biology volume 69, issue 6, page 1595-1611 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.2006.01226.x |
container_title |
Journal of Fish Biology |
container_volume |
69 |
container_issue |
6 |
container_start_page |
1595 |
op_container_end_page |
1611 |
_version_ |
1802639233611988992 |