In vitro interactions between Neoparamoeba spp. and salmonid leucocytes; the effect of parasite sonicate on anterior kidney leucocyte function

• Published in: Journal of Fish Biology
• Main Authors: Gross, K., Alcorn, S., Murray, A., Morrison, R., Nowak, B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2006
• Subjects: Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1095-8649.2006.01188.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2006.01188.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2006.01188.x

Sonicated Neoparamoeba spp. (Nspp) did not affect the in vitro respiratory burst response of leucocytes isolated from Atlantic salmon Salmo salar , rainbow trout Oncorhynchus mykiss and chinook salmon Oncorhynchus tshawytscha anterior kidneys ( P > 0·05). Atlantic salmon and chinook salmon leucocytes pre‐incubated with the parasites, however, responded to phorbol myristate acetate (PMA) stimulation with a greater response compared to cells incubated with PMA on its own ( P < 0·05). Sonicated Nspp was not chemo‐attractive for anterior kidney leucocytes isolated from all three fish species.