Feeding increases MyoD and PCNA expression in myogenic progenitor cells of Notothenia coviiceps

• Published in: Journal of Fish Biology
• Main Authors: Brodeur, J. C., Peck, L. S., Johnston, I. A.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2002
• Subjects: Antarctic; The Antarctic; Antarc*
• Online Access: http://dx.doi.org/10.1111/j.1095-8649.2002.tb02441.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2002.tb02441.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2002.tb02441.x

Oxygen consumption and ammonia excretion of the Antarctic fish Notothenia coriiceps (18.4 cm L F ) increased respectively two and fourfold above fasting levels 24 h after feeding with a single meal of shrimps (5.5 to 7.5% of body mass), and remained elevated for 120 h. In fasted fish, c‐met positive cells in the fast muscle represented 5.5% of the total number of myonuclei. The number of c‐met positive cells staining for the proliferating cell nuclear antigen was increased by 60% both 24 and 96 h after the meal, while the number of cells expressing the myogenic transcription factor, MyoD, was increased by 20% after 24 h, and by 44% after 96 h. The total numbers of c‐met positive cells and cells expressing myogenic were not significantly altered 96 h following feeding. The results are consistent with an activation of myogenic progenitor cells proliferation by feeding but suggest a relatively long cell cycle time.