Phagocytosis and giant cell formation at 0° C by macrophage (MØ) of Notothenia coriiceps

• Published in: Journal of Fish Biology
• Main Authors: Silva, J. R. M. C., Staines, N. A., Hernandez‐Blazquez, F. J., Porto‐Neto, L. R., Borges, J. C. S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2002
• Subjects: Admiralty Bay; Antarctic; Calmette; King George Island; Antarc*
• Online Access: http://dx.doi.org/10.1111/j.1095-8649.2002.tb00294.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.2002.tb00294.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.2002.tb00294.x

Macrophages adhered to round glass coverslips that had been implanted into the abdominal cavity of Notothenia coriiceps , collected from Admiralty Bay, King George Island, and removed and incubated in vitro for 4 h at 0° C at various intervals. The macrophages phagocytosed yeast Saccharomyces cerevisiae cells suspended in saline. Bi‐nuclear macrophages were first observed after 6 h. The formation of giant cells and their phagocytic activity was observed only in fish which had been injected with 1 ml of Bacillus of Calmette and Guerin (BCG)‐suspension 24 h before coverslip implantation and the coverslips removed 15 days after the implantation. Phagocytosis and the formation of giant cells in an Antarctic fish at 0° C is described for the first time.