Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny
At hatching, the yolk‐sac matrix of Siberian sturgeon Acipenser baeri contained neutral glycoconjugates, glycogen, proteins rich in arginine, lysine, tyrosine, cysteine and cystine, glycoproteins containing mannose (Man) and/or glucose (Glc), N ‐acetyl‐D‐galactosamine (GalNAc), L‐fucose (Fuc), siali...
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crwiley:10.1111/j.1095-8649.1999.tb00702.x 2024-06-02T08:14:23+00:00 Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny Gisbert, E. Sarasquete, M. C. Williot, P. Castelló‐Orvay, F. 1999 http://dx.doi.org/10.1111/j.1095-8649.1999.tb00702.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1999.tb00702.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1999.tb00702.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 55, issue 3, page 596-616 ISSN 0022-1112 1095-8649 journal-article 1999 crwiley https://doi.org/10.1111/j.1095-8649.1999.tb00702.x 2024-05-03T11:23:53Z At hatching, the yolk‐sac matrix of Siberian sturgeon Acipenser baeri contained neutral glycoconjugates, glycogen, proteins rich in arginine, lysine, tyrosine, cysteine and cystine, glycoproteins containing mannose (Man) and/or glucose (Glc), N ‐acetyl‐D‐galactosamine (GalNAc), L‐fucose (Fuc), sialic acid and/or N ‐acetyl‐D‐glucosamine (GlcNAc) residues, as well as neutral and acidic lipids. Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP ‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP ‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium. Article in Journal/Newspaper Siberian sturgeon Wiley Online Library Journal of Fish Biology 55 3 596 616 |
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Wiley Online Library |
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crwiley |
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English |
description |
At hatching, the yolk‐sac matrix of Siberian sturgeon Acipenser baeri contained neutral glycoconjugates, glycogen, proteins rich in arginine, lysine, tyrosine, cysteine and cystine, glycoproteins containing mannose (Man) and/or glucose (Glc), N ‐acetyl‐D‐galactosamine (GalNAc), L‐fucose (Fuc), sialic acid and/or N ‐acetyl‐D‐glucosamine (GlcNAc) residues, as well as neutral and acidic lipids. Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP ‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP ‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium. |
format |
Article in Journal/Newspaper |
author |
Gisbert, E. Sarasquete, M. C. Williot, P. Castelló‐Orvay, F. |
spellingShingle |
Gisbert, E. Sarasquete, M. C. Williot, P. Castelló‐Orvay, F. Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
author_facet |
Gisbert, E. Sarasquete, M. C. Williot, P. Castelló‐Orvay, F. |
author_sort |
Gisbert, E. |
title |
Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
title_short |
Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
title_full |
Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
title_fullStr |
Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
title_full_unstemmed |
Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny |
title_sort |
histochemistry of the development of the digestive system of siberian sturgeon during early ontogeny |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1999.tb00702.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1999.tb00702.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1999.tb00702.x |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
Journal of Fish Biology volume 55, issue 3, page 596-616 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1999.tb00702.x |
container_title |
Journal of Fish Biology |
container_volume |
55 |
container_issue |
3 |
container_start_page |
596 |
op_container_end_page |
616 |
_version_ |
1800738219585699840 |