Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod
Several nuclear RFLP loci have been discovered recently that exhibit extensive allele frequency variation among Norwegian coastal and north‐east Arctic populations of Atlantic cod Gadus morhua . One of these polymorphisms was detected by hybridizing an anonymous cDNA clone (GM798) against genomic DN...
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Online Access: | http://dx.doi.org/10.1111/j.1095-8649.1997.tb01529.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1997.tb01529.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1997.tb01529.x |
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crwiley:10.1111/j.1095-8649.1997.tb01529.x 2024-06-02T08:01:00+00:00 Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod Fevolden, S. E. Pogson, G. H. 1997 http://dx.doi.org/10.1111/j.1095-8649.1997.tb01529.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1997.tb01529.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1997.tb01529.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 51, issue 5, page 895-908 ISSN 0022-1112 1095-8649 journal-article 1997 crwiley https://doi.org/10.1111/j.1095-8649.1997.tb01529.x 2024-05-03T11:38:02Z Several nuclear RFLP loci have been discovered recently that exhibit extensive allele frequency variation among Norwegian coastal and north‐east Arctic populations of Atlantic cod Gadus morhua . One of these polymorphisms was detected by hybridizing an anonymous cDNA clone (GM798) against genomic DNA digested with the restriction enzyme Dra I. This cDNA clone has now been sequenced and identified as synaptophysin ( Syp I), an integral synaptic vesicle membrane protein. Primers were constructed that amplify an intron of the Syp I gene that is polymorphic for the Dra I site, thus making it possible to use a PCR‐based assay to score the polymorphism. A total of 965 individuals sampled from the Barents Sea, coastal areas and fjords in northern Norway have been analysed for this polymorphism. The results confirm that highly significant differences exist between NE Arctic and coastal cod at the Syp I locus. A cluster analysis revealed a deep split between coastal and Arctic populations and hierarchical F ‐statistics indicated that about 40% of the total variation was attributable to differences between Arctic and coastal groups. The temporal stability of allele frequencies was assessed by comparing Syp I allele frequencies among samples of juveniles (0 group) captured at specific locations in fjords in consecutive years and among samples of adults and juveniles collected from the same fjord. Samples of juveniles collected in 1994 and 1995 in Malangen were genetically indistinguishable whereas juveniles sampled from Dønnesfjord and Ullsfjorden over the same 2‐year period exhibited significant differences. Adults and 0‐group individuals collected from the same fjord were found to be genetically indistinguishable in Malangen, but not in Balsfjorden. In addition to detecting large differences among Arctic and coastal groups, the Syp I locus suggests that genetic heterogeneity exists among resident populations of cod in different fjords and that gene flow among populations throughout northern Norway may be considerably ... Article in Journal/Newspaper Arctic atlantic cod Balsfjorden Barents Sea Gadus morhua Malangen Northern Norway Wiley Online Library Arctic Balsfjorden ENVELOPE(19.014,19.014,69.420,69.420) Barents Sea Dønnesfjord ENVELOPE(22.623,22.623,70.650,70.650) Malangen ENVELOPE(18.598,18.598,69.400,69.400) Norway Journal of Fish Biology 51 5 895 908 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Several nuclear RFLP loci have been discovered recently that exhibit extensive allele frequency variation among Norwegian coastal and north‐east Arctic populations of Atlantic cod Gadus morhua . One of these polymorphisms was detected by hybridizing an anonymous cDNA clone (GM798) against genomic DNA digested with the restriction enzyme Dra I. This cDNA clone has now been sequenced and identified as synaptophysin ( Syp I), an integral synaptic vesicle membrane protein. Primers were constructed that amplify an intron of the Syp I gene that is polymorphic for the Dra I site, thus making it possible to use a PCR‐based assay to score the polymorphism. A total of 965 individuals sampled from the Barents Sea, coastal areas and fjords in northern Norway have been analysed for this polymorphism. The results confirm that highly significant differences exist between NE Arctic and coastal cod at the Syp I locus. A cluster analysis revealed a deep split between coastal and Arctic populations and hierarchical F ‐statistics indicated that about 40% of the total variation was attributable to differences between Arctic and coastal groups. The temporal stability of allele frequencies was assessed by comparing Syp I allele frequencies among samples of juveniles (0 group) captured at specific locations in fjords in consecutive years and among samples of adults and juveniles collected from the same fjord. Samples of juveniles collected in 1994 and 1995 in Malangen were genetically indistinguishable whereas juveniles sampled from Dønnesfjord and Ullsfjorden over the same 2‐year period exhibited significant differences. Adults and 0‐group individuals collected from the same fjord were found to be genetically indistinguishable in Malangen, but not in Balsfjorden. In addition to detecting large differences among Arctic and coastal groups, the Syp I locus suggests that genetic heterogeneity exists among resident populations of cod in different fjords and that gene flow among populations throughout northern Norway may be considerably ... |
format |
Article in Journal/Newspaper |
author |
Fevolden, S. E. Pogson, G. H. |
spellingShingle |
Fevolden, S. E. Pogson, G. H. Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
author_facet |
Fevolden, S. E. Pogson, G. H. |
author_sort |
Fevolden, S. E. |
title |
Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
title_short |
Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
title_full |
Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
title_fullStr |
Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
title_full_unstemmed |
Genetic divergence at the synaptophysin ( Syp I) locus among Norwegian coastal and north‐east Arctic populations of Atlantic cod |
title_sort |
genetic divergence at the synaptophysin ( syp i) locus among norwegian coastal and north‐east arctic populations of atlantic cod |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1997.tb01529.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1997.tb01529.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1997.tb01529.x |
long_lat |
ENVELOPE(19.014,19.014,69.420,69.420) ENVELOPE(22.623,22.623,70.650,70.650) ENVELOPE(18.598,18.598,69.400,69.400) |
geographic |
Arctic Balsfjorden Barents Sea Dønnesfjord Malangen Norway |
geographic_facet |
Arctic Balsfjorden Barents Sea Dønnesfjord Malangen Norway |
genre |
Arctic atlantic cod Balsfjorden Barents Sea Gadus morhua Malangen Northern Norway |
genre_facet |
Arctic atlantic cod Balsfjorden Barents Sea Gadus morhua Malangen Northern Norway |
op_source |
Journal of Fish Biology volume 51, issue 5, page 895-908 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1997.tb01529.x |
container_title |
Journal of Fish Biology |
container_volume |
51 |
container_issue |
5 |
container_start_page |
895 |
op_container_end_page |
908 |
_version_ |
1800745241386418176 |