Genetic analysis of population structure of tusk in the North Atlantic

• Published in: Journal of Fish Biology
• Main Authors: Johansen, T., Nævdal, G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1995
• Subjects: Faroe Islands; Greenland; Rockall Bank; Storegga; Davis Strait; East Greenland; North Atlantic
• Online Access: http://dx.doi.org/10.1111/j.1095-8649.1995.tb06058.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1995.tb06058.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1995.tb06058.x

To study the population structure of tusk Brosme brosme in the North Atlantic, samples from six fishing grounds were collected: St Kilda; Storegga; Faroe Islands; Rockall bank; Davis Strait and East Greenland. In addition two samples from the Norwegian coast; Kragerø and Øygarden, were included. Nine enzymes and haemoglobin were analysed by starch gel and agar gel electrophoresis. In addition isoelectric focusing (IEF) was carried out. At least 22 loci were represented. Polymorphisms were shown in eight loci and average heterozygosity was found to be about 0.05. Highest heterozygosity was found in haemoglobin analysed by Smithies buffer pH 8.6. Malate dehydrogenase ( MDH‐2 ) analysed in IEF (pH 3.5‐9.5), and haemoglobin were found as valuable tools for studies of stock structure in tusk. In the Greenland samples only one of the MDH‐2 heterozygotes was found, while in the East Atlantic both the observed heterozygotes were more frequent. The frequencies of the the Hb * b allele were about 0.40 and 0.70 in the samples from east and west Atlantic respectively.