Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon
This study investigates the patterns of genetic diversity detected in allozymes, mtDNA, and microsatellites, in order to assess their relative efficacy to differentiate sympatric landlocked salmon populations and to estimate changes in genetic diversity between wild and first‐generation hatchery fis...
Published in: | Journal of Fish Biology |
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Language: | English |
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crwiley:10.1111/j.1095-8649.1995.tb06051.x 2024-06-02T08:03:40+00:00 Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon Tessier, N. Bernatchez, L. Presa, P. Angers, B. 1995 http://dx.doi.org/10.1111/j.1095-8649.1995.tb06051.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1995.tb06051.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1995.tb06051.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 47, issue sA, page 156-163 ISSN 0022-1112 1095-8649 journal-article 1995 crwiley https://doi.org/10.1111/j.1095-8649.1995.tb06051.x 2024-05-03T12:00:12Z This study investigates the patterns of genetic diversity detected in allozymes, mtDNA, and microsatellites, in order to assess their relative efficacy to differentiate sympatric landlocked salmon populations and to estimate changes in genetic diversity between wild and first‐generation hatchery fish. Overall, the three genetic markers indicated a genetic differentiation between two sympatric populations of Lake Saint‐Jean, Québec. MtDNA and microsatellites also showed significant differences between wild and first‐generation hatchery fish originating from the same river. Allozyme analysis was the most limited approach due to the low genetic diversity detected and the necessity to kill specimens. Although low polymorphism was found in mtDNA, it was the most discriminant marker between wild populations. Microsatellite analysis appears to be a promising approach due to its high sensitivity in differentiating wild populations, in detecting changes in allele composition between wild and first‐generation hatchery fish and its potential for increased resolution by augmenting the number of polymorphic loci. Given the benefits and disadvantages of the three methods, the combination of mtDNA and microsatellite analyses will best address our research objectives. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Fish Biology 47 sa 156 163 |
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Wiley Online Library |
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language |
English |
description |
This study investigates the patterns of genetic diversity detected in allozymes, mtDNA, and microsatellites, in order to assess their relative efficacy to differentiate sympatric landlocked salmon populations and to estimate changes in genetic diversity between wild and first‐generation hatchery fish. Overall, the three genetic markers indicated a genetic differentiation between two sympatric populations of Lake Saint‐Jean, Québec. MtDNA and microsatellites also showed significant differences between wild and first‐generation hatchery fish originating from the same river. Allozyme analysis was the most limited approach due to the low genetic diversity detected and the necessity to kill specimens. Although low polymorphism was found in mtDNA, it was the most discriminant marker between wild populations. Microsatellite analysis appears to be a promising approach due to its high sensitivity in differentiating wild populations, in detecting changes in allele composition between wild and first‐generation hatchery fish and its potential for increased resolution by augmenting the number of polymorphic loci. Given the benefits and disadvantages of the three methods, the combination of mtDNA and microsatellite analyses will best address our research objectives. |
format |
Article in Journal/Newspaper |
author |
Tessier, N. Bernatchez, L. Presa, P. Angers, B. |
spellingShingle |
Tessier, N. Bernatchez, L. Presa, P. Angers, B. Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
author_facet |
Tessier, N. Bernatchez, L. Presa, P. Angers, B. |
author_sort |
Tessier, N. |
title |
Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
title_short |
Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
title_full |
Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
title_fullStr |
Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
title_full_unstemmed |
Gene diversity analysis of mitochondrial DNA, microsatellites and allozymes in landlocked Atlantic salmon |
title_sort |
gene diversity analysis of mitochondrial dna, microsatellites and allozymes in landlocked atlantic salmon |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1995.tb06051.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1995.tb06051.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1995.tb06051.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Biology volume 47, issue sA, page 156-163 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1995.tb06051.x |
container_title |
Journal of Fish Biology |
container_volume |
47 |
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sa |
container_start_page |
156 |
op_container_end_page |
163 |
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1800748259796320256 |