Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar)
The catabolic fate of circulating collagen (Col) in the Atlantic salmon ( Salmo salar ) was studied. Serum t 1/2 and organ distribution of circulating Col in salmon were determined using Col conjugated with l25 I‐tyramine cellobiose ( 125 I‐TC), a low molecular weight adduct which is trapped intraly...
Published in: | Journal of Fish Biology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1993
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1095-8649.1993.tb00328.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1993.tb00328.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1993.tb00328.x |
id |
crwiley:10.1111/j.1095-8649.1993.tb00328.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1095-8649.1993.tb00328.x 2024-09-15T17:56:23+00:00 Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) Smedsrød, B. Gjøent, T. Sveinbjørnsson, B. Berg, T. 1993 http://dx.doi.org/10.1111/j.1095-8649.1993.tb00328.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1993.tb00328.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1993.tb00328.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 42, issue 2, page 279-291 ISSN 0022-1112 1095-8649 journal-article 1993 crwiley https://doi.org/10.1111/j.1095-8649.1993.tb00328.x 2024-08-20T04:15:50Z The catabolic fate of circulating collagen (Col) in the Atlantic salmon ( Salmo salar ) was studied. Serum t 1/2 and organ distribution of circulating Col in salmon were determined using Col conjugated with l25 I‐tyramine cellobiose ( 125 I‐TC), a low molecular weight adduct which is trapped intralysosomally at the site of uptake. Intravenously administered l25 I‐tyramine cellobioselabelled Col type I was prepared either from salmon skin (sCol) or rat skin (rCol). Biphasic clearance kinetics of l25 I‐TC‐sCol in salmon were apparent, with 78% being removed from the circulation in an initial rapid α‐phase (t 1/2 (α) = 2.4 min), and 22% being removed more slowly in a terminalβ‐phase (t]2(β) = 25.8 min). Serum half life of 125 I‐TC‐rCol was found to be 5.4 min (in this type of experiment the number of data points allow the determination of only a monophasic decay slope). Approximately 90% of recovered radioactivity was found in the kidney of the fish. In comparative experiments, 74% of administered 125 I‐TC‐sCol was cleared from the circulation of rats during an initial rapid α‐phase with t l/2 (α) = 0.8 min, and 26% was eliminated in the terminal β‐phase with t 1/2 (β) = 7.2 min l25 I‐sCol was endocytosed and degraded in pure cultures of ral liver endothelial cells, which are the main site of clearance of circulating Col in the rat. Moreover, Col from the two species competed for the same receptor on cultured rat liver endothelial cells, Intravenous administration of tetramethyl rhodamine isothiocyanate‐labelled sCol (TRITC‐sCol) in salmon, and subsequent examination of sections of kidney in the fluorescence microscope, revealed that the fluorochrome was accumulated exclusively in discrete vesicles of sinusoidal lining cells. Analyses of kidney tissue 24h after intravenous administration of a mixture of fluorescein isothiocyanate‐labelled latex beads and TRITC‐sCol revealed no codistribution of the two fluorochromes, suggesting that the injected Col was taken up in cells different from macrophages. Purified ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Biology 42 2 279 291 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The catabolic fate of circulating collagen (Col) in the Atlantic salmon ( Salmo salar ) was studied. Serum t 1/2 and organ distribution of circulating Col in salmon were determined using Col conjugated with l25 I‐tyramine cellobiose ( 125 I‐TC), a low molecular weight adduct which is trapped intralysosomally at the site of uptake. Intravenously administered l25 I‐tyramine cellobioselabelled Col type I was prepared either from salmon skin (sCol) or rat skin (rCol). Biphasic clearance kinetics of l25 I‐TC‐sCol in salmon were apparent, with 78% being removed from the circulation in an initial rapid α‐phase (t 1/2 (α) = 2.4 min), and 22% being removed more slowly in a terminalβ‐phase (t]2(β) = 25.8 min). Serum half life of 125 I‐TC‐rCol was found to be 5.4 min (in this type of experiment the number of data points allow the determination of only a monophasic decay slope). Approximately 90% of recovered radioactivity was found in the kidney of the fish. In comparative experiments, 74% of administered 125 I‐TC‐sCol was cleared from the circulation of rats during an initial rapid α‐phase with t l/2 (α) = 0.8 min, and 26% was eliminated in the terminal β‐phase with t 1/2 (β) = 7.2 min l25 I‐sCol was endocytosed and degraded in pure cultures of ral liver endothelial cells, which are the main site of clearance of circulating Col in the rat. Moreover, Col from the two species competed for the same receptor on cultured rat liver endothelial cells, Intravenous administration of tetramethyl rhodamine isothiocyanate‐labelled sCol (TRITC‐sCol) in salmon, and subsequent examination of sections of kidney in the fluorescence microscope, revealed that the fluorochrome was accumulated exclusively in discrete vesicles of sinusoidal lining cells. Analyses of kidney tissue 24h after intravenous administration of a mixture of fluorescein isothiocyanate‐labelled latex beads and TRITC‐sCol revealed no codistribution of the two fluorochromes, suggesting that the injected Col was taken up in cells different from macrophages. Purified ... |
format |
Article in Journal/Newspaper |
author |
Smedsrød, B. Gjøent, T. Sveinbjørnsson, B. Berg, T. |
spellingShingle |
Smedsrød, B. Gjøent, T. Sveinbjørnsson, B. Berg, T. Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
author_facet |
Smedsrød, B. Gjøent, T. Sveinbjørnsson, B. Berg, T. |
author_sort |
Smedsrød, B. |
title |
Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
title_short |
Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
title_full |
Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
title_fullStr |
Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
title_full_unstemmed |
Catabolism of circulating collagen in the Atlantic salmon ( Salmo salar) |
title_sort |
catabolism of circulating collagen in the atlantic salmon ( salmo salar) |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1993.tb00328.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1993.tb00328.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1993.tb00328.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Biology volume 42, issue 2, page 279-291 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1993.tb00328.x |
container_title |
Journal of Fish Biology |
container_volume |
42 |
container_issue |
2 |
container_start_page |
279 |
op_container_end_page |
291 |
_version_ |
1810432589275594752 |