A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L.
We used the polymerase chain reaction (PCR) and direct DNA sequencing to study genetic variation within and among populations of Atlantic cod, Gadus morhua , in the western North Atlantic. In a 307 bp region of the mitochondrial cytochrome b gene, 24 variable nucleotide positions define 24 genotypes...
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Language: | English |
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1991
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Online Access: | http://dx.doi.org/10.1111/j.1095-8649.1991.tb05073.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1991.tb05073.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1991.tb05073.x |
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crwiley:10.1111/j.1095-8649.1991.tb05073.x 2024-06-02T08:03:09+00:00 A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. Carr, S. M. Marshall, H. D. 1991 http://dx.doi.org/10.1111/j.1095-8649.1991.tb05073.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1991.tb05073.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1991.tb05073.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 39, issue sA, page 101-107 ISSN 0022-1112 1095-8649 journal-article 1991 crwiley https://doi.org/10.1111/j.1095-8649.1991.tb05073.x 2024-05-03T12:07:12Z We used the polymerase chain reaction (PCR) and direct DNA sequencing to study genetic variation within and among populations of Atlantic cod, Gadus morhua , in the western North Atlantic. In a 307 bp region of the mitochondrial cytochrome b gene, 24 variable nucleotide positions define 24 genotypes, which differ by from one to six nucleotide substitutions. Greenland cod ( G. ogac ) differs from the most similar G. morhua genotype by an additional 12 nucleotide substitutions. Silent transitions dominate both intra‐ and interspecific comparisons, however four nucleotide substitutions within morhua result in amino acid replacements. Direct sequencing of DNA reveals substantially more of the genetic variation that exists within and between species than do previous indirect methods based on restriction fragment length polymorphisms, and thus has far greater potential to quantify such differences as may exist among fish stocks. Preliminary experiments also indicate that automation of DNA sequencing provides an efficient, rapid, and accurate means for detection of genetic variation in natural populations offish. Article in Journal/Newspaper atlantic cod Gadus morhua Greenland Greenland cod North Atlantic Wiley Online Library Greenland Journal of Fish Biology 39 101 107 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
We used the polymerase chain reaction (PCR) and direct DNA sequencing to study genetic variation within and among populations of Atlantic cod, Gadus morhua , in the western North Atlantic. In a 307 bp region of the mitochondrial cytochrome b gene, 24 variable nucleotide positions define 24 genotypes, which differ by from one to six nucleotide substitutions. Greenland cod ( G. ogac ) differs from the most similar G. morhua genotype by an additional 12 nucleotide substitutions. Silent transitions dominate both intra‐ and interspecific comparisons, however four nucleotide substitutions within morhua result in amino acid replacements. Direct sequencing of DNA reveals substantially more of the genetic variation that exists within and between species than do previous indirect methods based on restriction fragment length polymorphisms, and thus has far greater potential to quantify such differences as may exist among fish stocks. Preliminary experiments also indicate that automation of DNA sequencing provides an efficient, rapid, and accurate means for detection of genetic variation in natural populations offish. |
format |
Article in Journal/Newspaper |
author |
Carr, S. M. Marshall, H. D. |
spellingShingle |
Carr, S. M. Marshall, H. D. A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
author_facet |
Carr, S. M. Marshall, H. D. |
author_sort |
Carr, S. M. |
title |
A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
title_short |
A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
title_full |
A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
title_fullStr |
A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
title_full_unstemmed |
A direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of Atlantic cod, Gadus morhua L. |
title_sort |
direct approach to the measurement of genetic variation in fish populations: applications of the polymerase chain reaction to studies of atlantic cod, gadus morhua l. |
publisher |
Wiley |
publishDate |
1991 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1991.tb05073.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1991.tb05073.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1991.tb05073.x |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
atlantic cod Gadus morhua Greenland Greenland cod North Atlantic |
genre_facet |
atlantic cod Gadus morhua Greenland Greenland cod North Atlantic |
op_source |
Journal of Fish Biology volume 39, issue sA, page 101-107 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1991.tb05073.x |
container_title |
Journal of Fish Biology |
container_volume |
39 |
container_start_page |
101 |
op_container_end_page |
107 |
_version_ |
1800747618623553536 |