Cry (preservation of spermatozoa of the whitefish Coregonus muksun Pallas

• Published in: Journal of Fish Biology
• Main Authors: And, J. Plironen, Hyvärinen, H.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1983
• Subjects: Muksun; Coregonus muksun
• Online Access: http://dx.doi.org/10.1111/j.1095-8649.1983.tb04736.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1983.tb04736.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1983.tb04736.x

The cryopreservation methods for whitefish, Coregonus muksun , spermatozoa were studied using six different extenders with ‐40°C and ‐196°C as storing temperatures. In 1980, the spermatozoa of individual fishes were frozen in dry ice, in vials containing three different media based on glycerol as cryoprotectant. The pure glycerol media resulted in a 20% average rate of fertilization when samples were stored at — 40°C. The thawing procedure did not affect fertilization. In 1981, the sperm of individual fishes was frozen in pellets and stored in liquid nitrogen. Cryopreserved semen thawed in 0·119M NaHCO 3 produced 29·6–87·7% eyed eggs. The differences were caused mainly by the type of extender. The highest rate of fertility was obtained using an extender consisting of 0·3 M glucose with 20% glycerol (87·7% eyed eggs; 98·6% eyed eggs when compared to the corresponding control). The same values, using the modified extender of Stoss (Stoss & Refstie, in press), were 38·7–82·4% and, on the average, 90% eyed eggs, respectively.