Storage of marine fish erythrocytes in liquid nitrogen
The preservation of erythrocytes from cod ( Gadus morhua ), saithe ( Pollachius virens ) and mackerel ( Scomber scombrus ) at −196° C was studied using dimethyl sulphoxide (DMSO) as a cryoprotectant. Erythrocyte recoveries of greater than 90% were obtained from all species and cod erythrocytes were...
Published in: | Journal of Fish Biology |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1978
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1095-8649.1978.tb03458.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1978.tb03458.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1978.tb03458.x |
id |
crwiley:10.1111/j.1095-8649.1978.tb03458.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1095-8649.1978.tb03458.x 2024-06-02T08:06:56+00:00 Storage of marine fish erythrocytes in liquid nitrogen Chao, L. P. Birkbeck, T. H. 1978 http://dx.doi.org/10.1111/j.1095-8649.1978.tb03458.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1978.tb03458.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1978.tb03458.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 13, issue 4, page 483-488 ISSN 0022-1112 1095-8649 journal-article 1978 crwiley https://doi.org/10.1111/j.1095-8649.1978.tb03458.x 2024-05-03T11:57:10Z The preservation of erythrocytes from cod ( Gadus morhua ), saithe ( Pollachius virens ) and mackerel ( Scomber scombrus ) at −196° C was studied using dimethyl sulphoxide (DMSO) as a cryoprotectant. Erythrocyte recoveries of greater than 90% were obtained from all species and cod erythrocytes were stored for eighteen months with insignificant lysis. Larger quantities of blood were stored by removal of plasma from citrated blood prior to the addition of DMSO solution, and by storage of pelleted frozen blood in aluminium canisters in liquid nitrogen. Maximum recoveries of washed intact erythrocytes required thawing of pellets in 125% DMSO solution and washing with buffer containing decreasing concentrations of DMSO. Washed erythrocytes kept at 4° for at least two days showed little haemolysis, were morphologically similar to fresh erythrocytes and equally susceptible to the δ‐haemolysin of Staphylococcus aureus . Article in Journal/Newspaper Gadus morhua Wiley Online Library Journal of Fish Biology 13 4 483 488 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The preservation of erythrocytes from cod ( Gadus morhua ), saithe ( Pollachius virens ) and mackerel ( Scomber scombrus ) at −196° C was studied using dimethyl sulphoxide (DMSO) as a cryoprotectant. Erythrocyte recoveries of greater than 90% were obtained from all species and cod erythrocytes were stored for eighteen months with insignificant lysis. Larger quantities of blood were stored by removal of plasma from citrated blood prior to the addition of DMSO solution, and by storage of pelleted frozen blood in aluminium canisters in liquid nitrogen. Maximum recoveries of washed intact erythrocytes required thawing of pellets in 125% DMSO solution and washing with buffer containing decreasing concentrations of DMSO. Washed erythrocytes kept at 4° for at least two days showed little haemolysis, were morphologically similar to fresh erythrocytes and equally susceptible to the δ‐haemolysin of Staphylococcus aureus . |
format |
Article in Journal/Newspaper |
author |
Chao, L. P. Birkbeck, T. H. |
spellingShingle |
Chao, L. P. Birkbeck, T. H. Storage of marine fish erythrocytes in liquid nitrogen |
author_facet |
Chao, L. P. Birkbeck, T. H. |
author_sort |
Chao, L. P. |
title |
Storage of marine fish erythrocytes in liquid nitrogen |
title_short |
Storage of marine fish erythrocytes in liquid nitrogen |
title_full |
Storage of marine fish erythrocytes in liquid nitrogen |
title_fullStr |
Storage of marine fish erythrocytes in liquid nitrogen |
title_full_unstemmed |
Storage of marine fish erythrocytes in liquid nitrogen |
title_sort |
storage of marine fish erythrocytes in liquid nitrogen |
publisher |
Wiley |
publishDate |
1978 |
url |
http://dx.doi.org/10.1111/j.1095-8649.1978.tb03458.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1978.tb03458.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1978.tb03458.x |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Journal of Fish Biology volume 13, issue 4, page 483-488 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1095-8649.1978.tb03458.x |
container_title |
Journal of Fish Biology |
container_volume |
13 |
container_issue |
4 |
container_start_page |
483 |
op_container_end_page |
488 |
_version_ |
1800751930028326912 |